WormMine: ExpressionPattern Expr16267

• Pattern: We generated a more complete drl-1p::GFP reporter by fusing GFP coding sequence to a 5.1 kb genomic fragment that includes the upstream intergenic region and intron 1 and co-injected it with pharynx marker myo-2p::tdTomato. drl-1p::GFP was expressed in the epidermis and intestine consistent with previous mRNA enrichment studies (Pauli et al. 2006; Spencer et al. 2011; Blazie et al. 2017; Han et al. 2017). These results suggest that the upstream intergenic region drives expression in the intestine and that intron 1 also permits expression in the epidermis. A prior study failed to observe intestinal expression with a drl-1 transcriptional reporter (Chamoli et al. 2014); however, this former construct lacked the intergenic region upstream from exon 1 that drives intestinal expression.
• Primary Identifier: Expr16267

2 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772
Epidermal layer.	hypodermis	epidermis	WBbt:0005733

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00017578	drl-1	F18F11.5	Caenorhabditis elegans

0 Life Stages