WormMine: ExpressionPattern Expr9235

• Primary Identifier: Expr9235
• Subcellular Localization: The subcellular localization of TAT-1 in the intestine was determined by expressing TAT-1::CFP under control of the intestine-specific promoter vha-6 (Pvha-6tat-1::yfp). TAT-1 was localized to both plasma membranes and intracellular tubular and vesicular structures and colocalized with CHAT-1. The intestinal tubular and vesicular localization pattern was also observed when the expression of TAT-1 was controlled by the endogenous promoter. To determine the identities of the cytosolic compartments labeled by TAT-1, mCHERRY fusions of different endocytic markers were coexpressed together with CHAT-1::GFP and TAT-1 (Pvha-6chat-1::gfp +Pvha-6tat-1). TAT-1 was included to ensure efficient ER export of CHAT-1::GFP; this combination is subsequently referred to as CHAT-1::GFP for simplicity. CHAT- 1::GFP displayed a tubular and vesicular staining pattern, which did not overlap with either the Golgi marker MANS or the lysosomal marker Lysotracker Red, indicating that CHAT-1 is not on the Golgi or mature lysosomes. No CHAT-1::GFP was found on the RAB-7-positive ring-like structures, suggesting that it is not enriched on late endosomes or early lysosomes. CHAT-1 partially overlapped with RAB-5 on punctate structures, but not on tubule-like structures that were negative for mCHERRY::RAB-5. Thus, a proportion of CHAT-1/ TAT-1 may localize to RAB-5-positive early endosomes. CHAT-1/TAT-1 associates with the tubular membrane of early endosomes and ERCs. CHAT-1::GFP overlapped with mRFP::RME-1 on basolateral tubulo-vesicular structures, indicating that CHAT-1 also localizes to RME-1- positive recycling endosomes.

1 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00013034	tat-1	Y49E10.11	Caenorhabditis elegans

0 Life Stages