WormMine: ExpressionPattern Expr2571

• Pattern: Early to comma stage embryos in line PS4246, which shows 98% transmission of the transgene in larvae and adults, were devoid of expression and expression in pretzel stage embryos was barely detectable. In both transgenic lines analyzed, the majority of L1 larvae and 100% of L2, L3, and L4 larvae and adults showed medium to high expression, with the highest levels of expression noted in older adults. GFP expression under regulation of the spl-1 immediate upstream region was pronounced and localized almost exclusively in the intestine. All cells of the intestine expressed spl-1. In line PS4246, which transmits the non-Dpy phenotype at 98%, most animals demonstrated a fluorescent signal in the region just posterior to the pharynx with no expression in the rest of the anterior gut. Strong expression was also observed in the posterior gut of most animals. No expression was detected in the gonad, bodywall muscle, pharynx, vulva, or epidermis. Although a low percentage of animals showed some expression in a pair of cells in the head, expression was too weak and variable to identify these cells. This expression pattern was consistent in worms transformed with nuclear localizing reporter constructs and non-nuclear constructs. In contrast, only weak and non-tissue-specific expression from the T07A9.1 promoter constructs was observed.
• Primary Identifier: Expr2571
• Remark: The intragenic region between spl-1 and its nearest 5' neighbor (hypothetical gene T07A9.1) is 521 nucleotides. Due to the small size of this region, Wormbase (available at www.wormbase.org) predicts that spl-1 may be a downstream gene in an operon with T07A9.1. Therefore, to evaluate gene expression from the spl-1 locus, GFP reporter constructs were created from both potential regulatory regions, which are located immediately 5' to spl-1 and to the large intragenic region upstream of T07A9.1.

2 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772
anterior-most body region containing the pharynx.	head		WBbt:0005739

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00004981	spl-1	Y66H1B.4	Caenorhabditis elegans

5 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024