WormMine: ExpressionPattern Expr3212

• Pattern: Expression of the transgenic reporter construct is gut-specific, can first be detected late in embryogenesis and shows anterior - posterior patterning that is, to a reasonable approximation, the same as that detected by the histochemical assay for endogenous PHO-1 activity. In ~70% of newly hatched larvae, reporter expression is undetectable in the six cells belonging to int1 and int2 but strong in more posterior cells of the intestine. In the remaining ~30% of transgenic larvae, low levels of reporter expression can be detected in the four int1 cells; reporter expression is only rarely detected in the two int2 cells or in any cells outside of the intestine. Expression in the most posterior pair of intestinal cells (int9) appears to be weaker and more variable than in anterior neighbors.
• Primary Identifier: Expr3212
• Remark: Given the technical limitations of the histochemical assay for acid phosphatase activity (e.g., incomplete permeabilization and potential diffusion of the reaction product), it is difficult to rule out a similar low level of endogenous PHO-1 activity in a fraction of int1 cells or to claim with certainty that endogenous PHO-1 activity does indeed extend completely to the gut posterior.

1 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00004020	pho-1	EGAP2.3	Caenorhabditis elegans

0 Life Stages