WormMine: ExpressionPattern Expr12158

• Pattern: cav-2::GFP fusions were expressed in the intestine of hermaphrodite and male wild-type animals. Expression was not detected in any other tissues. This pattern was confirmed with an anti-CAV-2 antibody.
• Primary Identifier: Expr12158
• Subcellular Localization: Both cav-2::gfp fusion proteins localized to the apical side of the intestinal cells, very close to or within the membrane. Expression was first detected in midstage embryos and continued thence forward. The expression at the apical membrane was highest and most evenly distributed in L1 and L2 animals. With maturation, levels of CAV-2 were highest at the anterior and posterior cells of the intestine. Interestingly, the labeling of the membrane was discontinuous. We also observed the presence of discrete, spherically shaped, CAV-2::GFP-positive vesicles (diameter, 1.6 um; n=30). These vesicles were most common at the posterior end of the gut and were distinct from autofluorescent gut granules (AFGGs), which are terminal endocytic lysosomes. We call these structures CAV-2 bodies, as their relationship to mammalian caveosomes is unknown.

1 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00000302	cav-2	C56A3.7	Caenorhabditis elegans

0 Life Stages