WormMine: ExpressionPattern Expr10041

• Pattern: In situ hybridizations of dissected gonads were performed to ascertain the precise spatial pattern of spe-44 expression. The fem- 3(gf) and fem-1(lf) mutations were used to restrict hermaphrodite gametogenesis to sperm or oocyte production, respectively. Abundant expression was first detected in fem-3(gf) animals during the L3 stage in the early meiotic germline. DAPI staining of the chromatin indicated that expression coincides with early pachytene of meiosis I. By contrast, spe-44 expression was undetectable in fem-1(lf) germlines at any stage of development. Thus, spe-44 expression is restricted to the sperm-producing germline at a time and place consistent with the regulation of sperm gene expression.
• Primary Identifier: Expr10041

2 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
cell line which early in development becomes differentiated from the remaining somatic cell line, and alone has the potential to undergo meiosis and form gametes.	germ line	germline	WBbt:0005784
Male haploid germ cell.	sperm	spermatozoa	WBbt:0006798

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00007732	spe-44	C25G4.4	Caenorhabditis elegans

3 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038