WormMine

WS295

Intermine data mining platform for C. elegans and related nematodes

Expression Pattern :

Pattern  GON-4::GFP was found in the nucleus. Furthermore, GON-4::GFP was found in the somatic gonadal precursor cells, Z1 and Z4, and their descendants from L1 until the early L3 stage. Expression was weak, but reproducible. During L4, GFP was observed in four nuclei of developing vulva, but not in other nongonadal cells. Intriguingly, GON-4::GFP was detected in a scattering of germ-line nuclei of rescued animals. In contrast to the somatic gonadal expression, which occurred during early gonadogenesis, germ-line expression started in the second half of L4 and continued in adulthood. Primary Identifier  Expr957
Remark  This gon-4::GFP translational fusion rescues gon-4(q519) mutants to fertility when incorporated into the extrachromosomal array qEx453. Nine other rescuing lines also exhibited the same pattern. Preliminary results with polyclonal antibodies raised to the C-terminal portion of GON-4 are consistent with expression of GON-4 in the nuclei of dissected germ lines in mid- to late L4s and adults. These same antibodies have not proven useful with whole mounts of early larvae, which require a different protocol for antibody staining. Expression of the gon-4::GFP transgene was silenced in both somatic gonad and germ line. Subcellular Localization  nucleus
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22 Anatomy Terms

Definition Name Synonym Primary Identifier
cell line which early in development becomes differentiated from the remaining somatic cell line, and alone has the potential to undergo meiosis and form gametes. germ line germline WBbt:0005784
organ producing either sperm or ova. gonad   WBbt:0005175
cell, posteior daughter of P6.p P6.pp   WBbt:0006988
cell, anterior daughter of P6.p P6.pa   WBbt:0006987
cell, posterior descendent of P7.p P7.pp   WBbt:0006984
cell, anterior descendent of P7.p P7.pa   WBbt:0006983
post-embryonic cell of pedigree P5.pa P5.pa   WBbt:0007252
cell, anterior daughter of P6.pp P6.ppa   WBbt:0006991
cell, posterior daughter of P6.pa P6.pap   WBbt:0006990
cell, anterior daughter of P6.pa P6.paa   WBbt:0006989
post-embryonic cell of pedigree P5.ppa P5.ppa   WBbt:0007256
post-embryonic cell of pedigree P5.pp P5.pp   WBbt:0007255
post-embryonic cell of pedigree P5.pap P5.pap   WBbt:0007254
post-embryonic cell of pedigree P5.paa P5.paa   WBbt:0007253
post-embryonic cell of pedigree P7.ppp P7.ppp   WBbt:0007266
post-embryonic cell of pedigree P7.ppa P7.ppa   WBbt:0007265
post-embryonic cell of pedigree P7.pap P7.pap   WBbt:0007264
post-embryonic cell of pedigree P6.ppp P6.ppp   WBbt:0007260
Posterior daughter cell of P5.pp, fuses into hermaphrodite vulD syncytium. P5.ppp lineage name: P5.ppp WBbt:0004477
Anterior daughter cell of P7.pa, fuses into hermaphrodite vulD syncytium. P7.paa lineage name: P7.paa WBbt:0004448
Somatic gonad precursor cell Z4 lineage name: MSappaap WBbt:0004574
Somatic gonad precursor cell Z1 lineage name: MSpppaap WBbt:0004577

1 Genes

WormBase Gene ID Gene Name Sequence Name Organism
WBGene00001653 gon-4 K04D7.5 Caenorhabditis elegans

0 Life Stages