WormMine: ExpressionPattern Expr3154

• Pattern: In hermaphrodites, DAZ-1 was detected only in the germline of L2 through adulthood. This observation was consistent with the results of in situ hybridization against daz-1 mRNA carried out previously (see Expr964). With a closer look, however, the gradient of the abundance of DAZ-1 protein within adult gonads did not coincide with that of the mRNA. The mRNA was most abundant in the pachytene region (see Expr964), but DAZ-1 protein was most abundant in the mitotic region and also in the transition zone, where germ cells enter meiosis. Progression of pachytene stage was accompanied by a decrease of DAZ-1 protein, and staining of DAZ-1 faded out around the middle of that stage. No fluorescent signal was detected in the gonads of daz-1-deficient hermaphrodites, confirming that the observed staining was specific to DAZ-1. In males, daz-1 mRNA was barely detectable by in situ hybridization, and loss of daz-1 function did not affect their fertility (Expr964). Rather unexpectedly, however, DAZ-1 protein was detected in the male germline. As in hermaphrodites, DAZ-1 staining was detectable in the mitotic region and the transition zone of adult male gonads, and also in the germline of L3 and L4 larvae. Quantification by Western blotting using the anti-DAZ-1 antibodies indicated that the amount of DAZ-1 protein per gonad of males was approximately 20% of that of hermaphrodites.
• Primary Identifier: Expr3154
• Subcellular Localization: An observation by confocal microscopy under high magnification revealed that DAZ-1 localized to the cytoplasm of syncytial germ cells and to the cytoplasmic core (rachis).

1 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
cell line which early in development becomes differentiated from the remaining somatic cell line, and alone has the potential to undergo meiosis and form gametes.	germ line	germline	WBbt:0005784

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00000935	daz-1	F56D1.7	Caenorhabditis elegans

4 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038