| Pattern | By immunocytochemistry, DCR-1 was present throughout both the C. elegans germline and the somatic tissue (most easily seen in the extruded intestine) in wild-type worms. | Primary Identifier | Expr11565 |
| Remark | Temporal description | Subcellular Localization | Throughout the germline, DCR-1 was detected at low levels in uniformly-distributed small granules, more diffusely throughout the cytoplasm, and in some P granules. In the meiotic pachytene region, DCR-1 was also concentrated in distinct foci inside the nucleus that were sometimes adjacent to puncta stained by mAb414, an antibody that recognizes several nucleoporin proteins; however, the degree of co-localization was not high, with a Pearson's coefficient of 0.26. Around pachytene nuclei, DCR-1 occasionally co-localized with GLH-1 in P granules but did not have a high degree of co-localization with GLH-1; here the Pearson's coefficient was 0.36. While the distribution of DCR-1 in germ cell nuclei included puncta on the inner face of nuclear pores, GLH-1 was almost exclusively perinuclear and highly enriched in P granules on the cytoplasmic side of nuclear pores. The Pearson's coefficient of 0.82 suggests a high degree of co-localization between GLH-1 and nuclear pores. Thus, although DCR-1 and GLH-1 do not appear to have a high degree of co-localization, the GLH-1/DCR-1 biochemical interaction may reflect a transient interaction at or near nuclear pores. While GLH-1 is highly enriched at nuclear pores, DCR-1 seems likely to traffic through the nuclear pores as it is detected both inside nuclei and in the cytoplasm. In maturing wild type oocytes, DCR-1 was also detected throughout the cytoplasm and nucleus, sometimes slightly enriched at plasma membranes. |
| Definition | Name | Synonym | Primary Identifier |
|---|---|---|---|
| cell line which early in development becomes differentiated from the remaining somatic cell line, and alone has the potential to undergo meiosis and form gametes. | germ line | germline | WBbt:0005784 |
| Nucleated cell which has a diploid set of chromosomes. | somatic cell | FMA:72300 | WBbt:0008378 |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00000939 | dcr-1 | K12H4.8 | Caenorhabditis elegans |
