WormMine: ExpressionPattern Expr2301

• Pattern: Further evidence for differential accumulation was obtained using quantitative real-time PCR analysis (QPCR) with appropriate transcript-specific primers The levels of the rare 7a transcripts increase over 100-fold from early embryos to adulthood. In addition, the QPCR results show that the levels of 7b mRNAs (unclear from the RNA blot because of non-uniform loading) are approximately the same in adult soma and complete adults, indicating that both 7b and 7a mRNAs are present in adult soma. The lower relative levels of 7a mRNAs in complete adults compared with adult soma raises the possibility that 7a mRNAs are not present in the hermaphrodite germline. RNA blot analysis of stage-specific total RNA with transcript-specific probes showed different accumulation patterns for the four unc-62 mRNAs. The 1a-containing mRNAs were more abundant than the 1b-containing mRNAs, but their temporal patterns of accumulation appeared similar. Both were present in adult germline, pregastrulation embryos, later embryos and adult soma. The 7a signals were too weak to assess relative abundances at different stages. The 7b-containing mRNAs appeared much more abundant than the 7a RNAs in both embryos and adults.
• Primary Identifier: Expr2301

1 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
cell line which early in development becomes differentiated from the remaining somatic cell line, and alone has the potential to undergo meiosis and form gametes.	germ line	germline	WBbt:0005784

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00006796	unc-62	T28F12.2	Caenorhabditis elegans

6 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021
	The C. elegans life stage spanning 350-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The stage that embryo starts elongation until elongation is over.		elongating embryo Ce	WBls:0000015
	The C. elegans life stage spanning 290-350min after first cleavage at 20 Centigrade. Proliferate from 421 cells to 560 cells. The stage when embryo just finished gastrulation and is enclosing.		enclosing embryo Ce	WBls:0000013
	The C. elegans life stage spanning 100-290min after first cleavage at 20 Centigrade. Proliferate from 28 cells to 421 cells. Referring to the whole period of gastrulation.		gastrulating embryo Ce	WBls:0000010
	The C. elegans life stage spanning 0-100min after first cleavage at 20 Centigrade. Proliferate from 1 cell to 28 cells. From first cleavage until the start of gastrulation.		blastula embryo Ce	WBls:0000005