WormMine: ExpressionPattern Expr2347

• Pattern: CYE-1 is present in adult animals and is restricted to the germline, which is the only proliferative tissue in adults. CYE-1 levels vary in the germline. Mitotic germ cells in the distal region of the gonad have easily detectable levels of nuclear CYE-1. Germ cells in the initial stages of meiosis (proximal to the mitotic germ cells) have lower CYE-1 levels. Finally, as oocytes cellularize in the loop region of the gonad, CYE-1 levels increase with mature oocytes having the highest levels of nuclear CYE-1. These results demonstrate that a significant portion of maternal cye-1 contribution to the embryo is CYE-1 protein. CYE-1 level was assayed postembryonically to determine whether CYE-1 could be detected and if levels of CYE-1 correlated with mitotic proliferation. CYE-1 protein is detectable in larval blast cells that give rise to all tissue types, including, germline, intestine, hypodermis, neurons, and muscle. During larval stages, the level of CYE-1 protein is much lower than that found in germ cells or in the early embryo. CYE-1 antibody staining is restricted to the developmental time when the blast cells are undergoing active proliferation. For example, in the L1 stage, proliferating P blast cells that produce ventral nerve cells have relatively high levels of nuclear CYE-1. In contrast, during the L2 larval stage, the nonproliferating neuronal descendents of the P blast cells have CYE-1 levels that are only barely detectable above background. Further, while a subset of the P cell descendents, the vulva precursor cells (VPCs), will proliferate in the L3 larval stage to produce the vulva, these cells do not have appreciable CYE-1 levels while they are quiescent in the L2 larval stage. Nuclear CYE-1 becomes detectable in the VPCs during the L3 larval stage when they begin proliferation. CYE-1 becomes undetectable in the VPC descendents after completion of cell divisions in L4 larval stage animals. Monoclonal anti-CYE-1 antibody was used to assay CYE-1 levels from fertilization to the end of embryogenesis. In the zygote, CYE-1 is observed in the maternal and paternal pronuclei as soon as they form. The specificity of antibody staining was confirmed by cye-1 RNAi treatment of adult hermaphrodites that abolishes both oocyte nuclei and embryonic anti-CYE-1 protein staining. In early embryos, CYE-1 is enriched in nuclei, and levels appear constant with no evidence of cell cycle fluctuations other than during mitosis. During mitosis, CYE-1 antibody staining appears diffuse once nuclear envelope breakdown occurs, but resumes nuclear localization upon reformation of the nuclear envelope in telophase. CYE-1 is present equally in all cells of the early embryo. The level of CYE-1 declines during embryogenesis and disappears from most cells in comma-stage embryos coincident with the completion of the majority of embryonic cell divisions.
• Primary Identifier: Expr2347
• Subcellular Localization: nuclear

41 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
cell line which early in development becomes differentiated from the remaining somatic cell line, and alone has the potential to undergo meiosis and form gametes.	germ line	germline	WBbt:0005784
The tenth (count from anterior-most) of twelve postembryonic blast cells for ventral cord motorneurons, ventral hypodermis, vulva, male preanal ganglion; ventral hypodermis in L1.	P10		WBbt:0006779
The ninth (count from anterior-most) of twelve postembryonic blast cells for ventral cord motorneurons, ventral hypodermis, vulva, male preanal ganglion; ventral hypodermis in L1.	P9		WBbt:0006778
Postembryonic blast cells for ventral cord motorneurons, ventral hypodermis, vulva, male preanal ganglion; ventral hypodermis in L1	P12	lineage name: ABprapappa	WBbt:0004409
Postembryonic blast cells for ventral cord motorneurons, ventral hypodermis, vulva, male preanal ganglion; ventral hypodermis in L1	P11	lineage name: ABplapappa	WBbt:0004410
Seam hypodermal cell, postembryonic blast cell	H1L	lineage name: ABplaaappp	WBbt:0004766
Seam hypodermal cell, postembryonic blast cell	H1R	lineage name: ABarpapppp	WBbt:0004765
Seam hyopdermal cell, postemb. blast cell, L1 deirid socket	H2L	lineage name: ABarppaaap	WBbt:0004764
Seam hyopdermal cell, postemb. blast cell, L1 deirid socket	H2R	lineage name: ABarpppaap	WBbt:0004763
The third (count from anterior-most) of twelve postembryonic blast cells for ventral cord motorneurons, ventral hypodermis, vulva, male preanal ganglion; ventral hypodermis in L1.	P3		WBbt:0006772
The second (count from anterior-most) of twelve postembryonic blast cells for ventral cord motorneurons, ventral hypodermis, vulva, male preanal ganglion; ventral hypodermis in L1.	P2		WBbt:0006771
The first (count from anterior-most) of twelve postembryonic blast cells for ventral cord motorneurons, ventral hypodermis, vulva, male preanal ganglion; ventral hypodermis in L1.	P1	P1 post-embryonic blast cell	WBbt:0006770
The seventh (count from anterior-most) of twelve postembryonic blast cells for ventral cord motorneurons, ventral hypodermis, vulva, male preanal ganglion; ventral hypodermis in L1.	P7		WBbt:0006776
The sixth (count from anterior-most) of twelve postembryonic blast cells for ventral cord motorneurons, ventral hypodermis, vulva, male preanal ganglion; ventral hypodermis in L1.	P6		WBbt:0006775
The fifth (count from anterior-most) of twelve postembryonic blast cells for ventral cord motorneurons, ventral hypodermis, vulva, male preanal ganglion; ventral hypodermis in L1.	P5		WBbt:0006774
The fourth (count from anterior-most) of twelve postembryonic blast cells for ventral cord motorneurons, ventral hypodermis, vulva, male preanal ganglion; ventral hypodermis in L1.	P4		WBbt:0006773
The eighth (count from anterior-most) of twelve postembryonic blast cells for ventral cord motorneurons, ventral hypodermis, vulva, male preanal ganglion; ventral hypodermis in L1.	P8		WBbt:0006777
Tail seam hypodermal cell, postembryonic blast cell, functions as phasmid socket in L1	TL	lineage name: ABplappppp	WBbt:0004946
Tail seam hypodermal cell, postembryonic blast cell, functions as phasmid socket in L1	TR	lineage name: ABprappppp	WBbt:0004944
Seam hypodermal cell, postembryonic blast cell	V1L	lineage name: ABarppapaa	WBbt:0004905
Seam hypodermal cell, postembryonic blast cell	V5R	lineage name: ABprapapaap	WBbt:0004876
Seam hypodermal cell, postembryonic blast cell	V6L	lineage name: ABarppappp	WBbt:0004875
Seam hypodermal cell, postembryonic blast cell	V6R	lineage name: ABarpppppp	WBbt:0004874
Seam hypodermal cell, postembryonic blast cell	V3R	lineage name: ABprappapa	WBbt:0004896
Seam hypodermal cell, postembryonic blast cell	V4L	lineage name: ABarppappa	WBbt:0004894
Seam hypodermal cell, postembryonic blast cell	V4R	lineage name: ABarpppppa	WBbt:0004892
Seam hypodermal cell, postembryonic blast cell	V5L	lineage name: ABplapapaap	WBbt:0004890
Seam hypodermal cell, postembryonic blast cell	V1R	lineage name: ABarppppaa	WBbt:0004904
Seam hypodermal cell, postembryonic blast cell	V2L	lineage name: ABarppapap	WBbt:0004902
Seam hypodermal cell, postembryonic blast cell	V2R	lineage name: ABarppppap	WBbt:0004900

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00000871	cye-1	C37A2.4	Caenorhabditis elegans

6 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003