WormMine: ExpressionPattern Expr12872

• Pattern: Green fluorescent protein (GFP) reporter constructs indicated that lin-46 is expressed in hypodermal cells, particularly the lateral and ventral hypodermis, in brief periods during larval development. Animals bearing a transcriptional fusion showed intense fluorescence in lateral hypodermal cells around the time of each molt, especially during the L2- and L4-lethargus. In L2 animals, not all seam cells fluoresced simultaneously, and often only a cluster of an even number of cells fluoresced in a given animal, suggesting the expression of the fusion is related to seam cell division, which occurs somewhat asynchronously during larval development (Sulston and Horvitz, 1977). A lin-46 translational fusion reporter showed a similar expression pattern, although it was less intense. The translational fusion also showed strong constitutive expression in the cell bodies and axons of the bilateral motor interneurons AVB, but we do not know the significance of this expression. The LIN-46:GFP fusion protein localized to both cytoplasm and nucleus, but mostly in the non-nucleolar part of the nucleus. Green fluorescent protein (GFP) reporter constructs indicated that lin-46 is expressed in hypodermal cells, particularly the lateral and ventral hypodermis, in brief periods during larval development. Animals bearing a transcriptional fusion showed intense fluorescence in lateral hypodermal cells around the time of each molt, especially during the L2- and L4-lethargus. In L2 animals, not all seam cells fluoresced simultaneously, and often only a cluster of an even number of cells fluoresced in a given animal, suggesting the expression of the fusion is related to seam cell division, which occurs somewhat asynchronously during larval development (Sulston and Horvitz, 1977).
• Primary Identifier: Expr12872
• Subcellular Localization: The translational fusion also showed strong constitutive expression in the cell bodies and axons of the bilateral motor interneurons AVB, but we do not know the significance of this expression. The LIN-46:GFP fusion protein localized to both cytoplasm and nucleus, but mostly in the non-nucleolar part of the nucleus.

2 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
Epidermal layer.	hypodermis	epidermis	WBbt:0005733
a group of hypodermal cells that lie along the apical midline of the hypodermis, at the extreme left and right sides between nose and tail	seam cell	lateral hypodermis	WBbt:0005753

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00003031	lin-46	R186.4	Caenorhabditis elegans

2 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage during L3-L4 molt when an animal becomes lethargic, ceases pharyngeal pumping and synthesizes new cuticle under the old.		L3-L4 lethargus Ce	WBls:0000036
	The stage during L2-L3 molt when an animal becomes lethargic, ceases pharyngeal pumping and synthesizes new cuticle under the old.		L2-L3 lethargus Ce	WBls:0000028