WormMine: ExpressionPattern Expr3754

• Pattern: At the pre-comma stage, at about 260 min of embryonic development, RNT-1::GFP expression began in the nuclei of the seam cells (H0-2, V1-6 and T cells). At the comma stage, at about 400 min, expression also appeared in the body wall muscle cells . At the L1 larval stage, RNT-1::GFP expression was observed in the seam cells, the body wall muscle cells, and occasionally in the intestinal cells. In the H, V, and T cell lineages, RNT-1::GFP expression was always observed in the seam (self-renewing) cells, but not in the hypodermal syncytial (differentiating) cells, from L1 to adult stages. In addition, RNT-1::GFP expression was observed in the descendants of the M blast cell from late L1 to adult stages. The expression began after two rounds of M cell division, and continued in the body wall muscle descendants. RNT-1::GFP was strongly expressed in the T cells until just before the initiation of mitosis. The expression was diminished in prophase and not visible from metaphase to telophase of the T cell division. The expression was not detectable in either daughter of the T cells, T.a and T.p, but reappeared in the posterior daughters of the T.a cells, T.ap, several hours after the T.a cell division in late L1 stage. After this stage, RNT-1::GFP expression was exclusively observed in T.app and T.appa, but not in either of the other T.ap descendants or any T.p descendants.
• Primary Identifier: Expr3754
• Remark: RNT-1::GFP expression was not observed in T.a and T.p by anti-GFP antibody staining to enhance the GFP signal intensity, or by cultivating worms at 15 centigrades for accumulation and maturation of GFP in T.a and T.p.
• Subcellular Localization: Expressed in the nuclei

2 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
Longitudinal bands of muscle cells surrounding animal body, with one band running in each quadrant of the body, regulated contraction and relaxation of these muscles cause locomotion.	body wall musculature	body muscle	WBbt:0005813
a group of hypodermal cells that lie along the apical midline of the hypodermis, at the extreme left and right sides between nose and tail	seam cell	lateral hypodermis	WBbt:0005753

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00004393	rnt-1	B0414.2	Caenorhabditis elegans

11 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021
	The C. elegans life stage spanning 390-420min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo looks like a comma. A stage between bean embryo and 1.5-fold embryo.		comma embryo Ce	WBls:0000017
	The C. elegans life stage spanning 460-520min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and double fold. A stage between 1.5-fold embryo and 3-fold embryo.		2-fold embryo Ce	WBls:0000019
	The C. elegans life stage spanning 520-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and tripple fold. A stage between 2-fold embryo and fully-elongated embryo. Also called pretzel embryo or pretzel stage.		3-fold embryo Ce	WBls:0000020
	The C. elegans life stage spanning 420-460min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and fold back 50%. A stage between comma embryo and 2-fold embryo.		1.5-fold embryo Ce	WBls:0000018
	The C. elegans life stage spanning 350-390min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. Emrbyo elongation started but have not formed comma shape yet. The shape of embryo looks like a lima bean. A stage right before comma embryo. Also called lima embryo or lima bean stage.		bean embryo Ce	WBls:0000016