WormMine: ExpressionPattern Expr16531

• Pattern: Endogenously tagged BLI collagens localize to puncta corresponding to cuticle struts. BLI-1::mNG fusion proteins were expressed exclusively in the late L4 epidermis and in the nascent and mature adult cuticle. Within the cuticle, BLI-1::mNG was localized to periodically repeating puncta of 0.25-0.3μm in diameter in cir- cumferential rows that extended from the dorsal or ventral midlines to the lateral alae. Based on their adult-specific expression and distribution, such puncta correspond to the cuticle struts originally observed in phase contrast and in TEM16, and are consistent with localization of an extrachromosomal transgenic BLI-1::GFP reporter31,33. BLI-1::mNG animals also expressed some diffuse mNG signal in the underlying epidermis beginning in the mid-L4 stage, presumably corresponding to BLI-1::mNG in the secretory pathway. XFP knock-ins within the BLI-1 N- and C-terminal domains (ju1789 and ju1791 respectively) displayed similar localization (Fig. S2a, b); most analysis below focused on the ju1789 N-terminal mNG knock-in in 1-2 day old adults.
• Primary Identifier: Expr16531

1 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
a rigid external coating which is secreted by the hypodermis, seam and some transitional epithelial cells which covers the outer body, the major openings into the body cavity from the exterior, and two large sensory bristles, the male spicules. The cuticle of the spicules, the hook, and of some portions of the spicule channels is especially rigid and is probably sclerotized. Similar sclerotic features are occasionally noted in the ventral surface of the male tail fan.	cuticle	exoskeleton	WBbt:0005755

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00000251	bli-1	C09G5.6	Caenorhabditis elegans

2 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038