WormMine: ExpressionPattern Expr1790

• Pattern: daf-9 was strikingly expressed in a ventral pair of bilateral neurons identified as the IL1Vs or URAVs in the anterior ganglia. Neuronal expression first appeared shortly before hatch, increased greatly during larval development, and perdured in reproductive and postreproductive adults. By mid-L2, DAF-9 also appeared in the cytoplasm of the hypodermis, the syncitial epidermis surrounding the worm, but was absent from midline epidermal seam cells. Levels peaked around the L2 molt and diminished during L4. In some cases, transient expression was seen in L3 vulval blast cells. daf-9 was also expressed within the hermaphrodite spermatheca starting in late L4 larvae, and continuing even in old adults. In some extrachromosomal lines, sporadic weak expression was seen in the excretory cell, a few unidentified head neurons, and vulval muscles. In males, daf-9 was expressed in IL1V/URAVs and hypodermis but not somatic gonad. In dauer larvae, daf-9 was strongly expressed in IL1V/URAV and specifically extended into axonal but not dendritic processes. No hypodermal expression was evident in dauers. In post-dauer stages, daf-9 was expressed in a pattern similar to reproductively growing animals, except expression was absent in the hypodermis.
• Primary Identifier: Expr1790
• Subcellular Localization: DAF-9 was perinuclear, consistent with residence in endoplasmic reticulum.

9 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
an accordion-like tube that contains sperm and is the site of oocyte fertilization.	spermatheca		WBbt:0005319
Epidermal layer.	hypodermis	epidermis	WBbt:0005733
muscle associated with hermaphrodite vulva.	vulval muscle		WBbt:0005821
neuron with its cell body situated in the head, excluding the pharynx.	head neuron		WBbt:0006751
H-shaped cell associated with the excretory system, largest cell in C. elegans.	excretory cell	excretory canal cell	WBbt:0005812
Inner labial neuron	IL1VL	lineage name: ABalppapppaa	WBbt:0004549
Inner labial neuron	IL1VR	lineage name: ABarapppppaa	WBbt:0004547
Ring motor neuron	URAVL	lineage name: ABplpaaapaa	WBbt:0004935
Ring motor neuron	URAVR	lineage name: ABprpaaapaa	WBbt:0004934

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00000905	daf-9	T13C5.1	Caenorhabditis elegans

10 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	A third stage larva specialized for dispersal and long term survival.		dauer larva Ce	WBls:0000032
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021
	A C. elegans L4 larval life stage that occurs after the animal has recovered from the dauer diapause.		post dauer L4 stage Ce	WBls:0000828
	A C. elegans adult life stage that occurs after the animal has recovered from the dauer diapause.		post dauer adult stage Ce	WBls:0000829
	The stage right after an animal recovered from dauer but has not started transformation to L4 larva yet.		post dauer L3 stage Ce	WBls:0000052