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Expr15024
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Consistent with the previous report on the hermaphrodites' expression, we confirmed here that for both sexes, all three of pck-1-expressing reporters express in head and body wall muscles. However, we found that the reporters also express in ventral cord and preanal neurons and nerve ring neurons per side in the head. In the tail region, both larval males and larval/adult hermaphrodites express the transgenes in the dorsal rectal ganglion and three neurons per side in the tail. The adult male shows additional expression in sex-specific neurons of the ray, postcloacal, and spicule sensilla. None of the constructs promote expression in male or hermaphrodite sex muscles, intestine, or pharyngeal muscles. |
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Reporter gene fusion type not specified. |
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Expr3362
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Expressed in male spicule neuron. |
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Expr3020
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Expressed in ADL, ASI, ASH, ASE, FLP, URA, MC, M4, M2. Male specific expression in P6-9, SP. Faint or variable expression observed in DVF. |
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Expr14524
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Pcdc-25.4::gfp was expressed in many neurons, including pharyngeal neurons, the ventral nerve cord, and tail neurons in both hermaphrodites and males. However, the signal was not detected in the dorsal nerve cord. Pcdc-25.4::gfp expression was also observed in pharyngeal muscles, the head ganglion, preanal ganglion, and lumbar ganglion. In particular, Pcdc-25.4::gfp was expressed in ray cell bodies, spicule neurons, hook neurons, and postcloacal sensilla neurons in male tails. cdc-25.4 was also expressed in germ cells, including oocytes and sperm. |
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Expr15831
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Expression analysis of a FLP-3 translational fusion (pflp-3::flp- 3::mCherry) confirmed previous expression analyses of the neuropeptide within male-specific spicule neurons. Transcriptional reporters have shown robust flp-3 expression in the amphid IL1 neurons, as well as the sensory PQR and the male-specific interneuron, CP9, although our translational fusion exhibited no PQR or CP9 expression. Previous studies employed 1-2kb regions of promoter sequence driving GFP expression,while our construct employs a 4 kb region, thereby incorporating further regulatory elements that may restrict expression patterns. By including the full coding sequence in our translational fusion, we have also incorporated the regulatory elements found within the introns of the flp-3 gene.Interestingly, we observed localization of mCherry within sensory cilia of male dorsal and ventral IL1 neurons, as well as in puncta spanning their dendrites, consistent with peptide packing into dense core vesicles. Previous studies have observed dense core vesicles within, and being released from, dendritic arbors, supporting our findings that FLP-3 is packaged within the dendrites of the IL1 neurons. We found an identical expression pattern within the IL1 cilia and dendritic puncta of hermaphrodites as well. In males, our flp-3 construct also exhibited male-specific tail expression in the SPD spicule neurons. |
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Expr3005
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Expressed in IL1 and PQR. Male specific expression in SP and CP9. |
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Expr15030
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In addition to male sex muscles, EGL-2::YFP is also expressed on the cell bodies, neural processes, and sensory endings of the male ray, post-cloacal, and spicule sensory neurons. the protractor muscles' EGL-2::YFP levels also increased between 24 and 48 h in wild-type males. |
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