WormMine: AnatomyTerm WBbt:0005832

• Definition: one of the two AWC neurons, stochastically determined, in which the str-2 promoter is active
• Name: AWC-ON
• Primary Identifier: WBbt:0005832

0 Children

1 Expression Clusters

Regulated By Treatment	Description	Algorithm	Primary Identifier
	Transcripts enriched in AWC_ON according to single cell RNAseq.	Genes that pass the Bonferroni threshold for multiple comparisons (q < 0.05) are significantly enriched.	WBPaper00061651:AWC_ON_enriched

37 Expression Patterns

• Primary Identifier: Expr16432
• Pattern: We detected DMD-9::GFP expression in 10 head neurons of hermaphrodites at the L4 stage. High dmd-9 expression was detected in the BAG, AFD, AWCon, AWCoff, ASEL, and AWB neurons, with lower expression in ASER, and the ASGs. We also observed weak and inconsistent expression in other head neurons and transient weak expression in the tail of hermaphrodites and males at the L4 stage. No overt differences were observed in DMD-9::GFP expression in neurons of hermaphrodites and males. We also detected DMD-9::GFP expression in non-neuronal tissues. Transient DMD-9::GFP expression was observed in the uterine cells at specific sub-stages of the L4 stage. DMD-9 is not expressed from L4.0 to L4.3, with expression first detected in the early L4.4 stage, with the highest level during L4.4. before gradual loss by the L4.9 stage (Mok et al. 2015). DMD-9::GFP was not detected in adult uterine cells. We also detected DMD-9::GFP expression in sperm of hermaphrodites and males. In addition, we found that in a small proportion of L4 animals DMD-9::GFP shows asymmetric expression in the ASEs and AWCs neurons.

• Primary Identifier: Expr15558

• Primary Identifier: Expr15567

• Primary Identifier: Expr15571

• Primary Identifier: Expr15572

• Primary Identifier: Expr15573

• Primary Identifier: Expr15579

• Primary Identifier: Expr15586

• Primary Identifier: Expr15651

• Primary Identifier: Expr15652

• Primary Identifier: Expr15589

• Primary Identifier: Expr15591

• Primary Identifier: Expr15598

• Primary Identifier: Expr15604

• Primary Identifier: Expr15606

• Primary Identifier: Expr15608

• Primary Identifier: Expr15611

• Primary Identifier: Expr15648

• Primary Identifier: Expr15575

• Primary Identifier: Expr15596

• Primary Identifier: Expr15716
• Pattern: Consistent with previous findings, only 1 of the AWC neurons expressed nsy-7p::GFP in those transgenic animals that displayed GFP expression in AWC cells. Asymmetric expression of nsy- 7p::GFP in AWCL or AWCR was stochastic. nsy-7 was exclusively expressed in AWCON neurons, while no expression was observed in AWCOFF cells.

• Primary Identifier: Expr15718
• Pattern: imb-2/Transportin 1 Is Asymmetrically Expressed in AWCON Neurons. imb- 2::mNG knock-in animals displayed wild-type AWC asymmetry, indicating that the tagged IMB-2::mNG fusion protein is functional. IMB-2::mNG was detected in the nucleus and around the nuclear envelope of numerous cells in the head and body during embryogenesis, larval stages, and adulthood. The broad expression pattern of imb-2 in the head is consistent with expression of vertebrate transportin 1 in multiple regions of the brain, including the olfactory bulb. IMB-2::mNG was detected in both AWC neurons in late embryogenesis during which AWC asymmetry was established and was maintained until adulthood. IMB-2::mNG was asymmetrically expressed in the left AWC neuron (AWCL) or the right AWC neuron (AWCR) in a stochastic manner. Stochastic asymmetry of imb-2 expression in AWC neurons is consistent with the random nature of AWC asymmetry. IMB-2::mNG was expressed at a significantly higher expression level in the AWCON neuron than the AWCOFF neuron in the majority of animals.

• Primary Identifier: Expr12699
• Pattern: slo-1 and slo-2 are expressed in both AWC neurons in the majority of wild-type animals, both slo-1 and slo-2 are asymmetrically expressed in AWCL or AWCR in a stochastic manner (AWCL>AWCR versus AWCL

• Primary Identifier: Expr10194
• Pattern: 52% of animals had visible mir-71p::YFP in both AWC cells, 28% had visible YFP in only AWC left (AWCL), and 20% had visible YFP in only AWC right (AWCR).bmir- 71p::GFP expression was significantly higher in the AWC-ON cell than in the AWC-OFF cell in 71% of the animals.

• Primary Identifier: Expr15376

• Primary Identifier: Expr15633

• Primary Identifier: Expr15584

• Primary Identifier: Expr14143
• Pattern: AWA, asymmetric expression in AWC ON

• Primary Identifier: Expr15617

• Primary Identifier: Expr15953
• Pattern: mNG::mls-2 knock-in animals displayed wild-type AWC asymmetry as determined by the expression of the AWCON marker str-2p::TagRFP, suggesting that mNG::MLS-2 fusion protein is functional in AWC development. Like GFP::MLS-2 expressed from transgenes, mNG::MLS-2 knock-in was localized in the nucleus of AWC precursor cells in early embryos but was not observed in AWC cells in late embryos or early-stage larvae. Similar to MLS-2 antibody staining and GFP::MLS-2 transgenes (Jiang et al., 2005), mNG::MLS-2 knock-in was localized to the nucleus of a subset of head cells and the M mesoblast in first-stage larvae and adults.

0 Life Stages

1 Parents

Definition	Name	Synonym	Primary Identifier
Neuron class of two ciliated neurons with large, flattened, sheet-like endings that are associated with the sheath cells of the amphid sensilla.	AWC		WBbt:0005672