WormMine: ExpressionPattern Expr2772

• Pattern: The body-wall muscle staining became apparent at the 3.5-fold stage of embryogenesis, was most apparent in late embryos and L1 stage larvae, and persisted to adulthood. The vulval muscles, predominantly the four Vm1 cells, and the intestinal muscles also displayed GFP fluorescence. Weaker fluorescence was observed in the anal depressor and anal sphincter muscles. GFP expression was also observed in 1215 head neurons in each animal, including in the SIADL, SIADR, SIAVL, and SIAVR neurons. The body-wall muscle staining became apparent at the 3.5-fold stage of embryogenesis, was most apparent in late embryos and L1 stage larvae, and persisted to adulthood. The vulval muscles, predominantly the four Vm1 cells, and the intestinal muscles also displayed GFP fluorescence. Weaker fluorescence was observed in the anal depressor and anal sphincter muscles. GFP expression was also observed in head neurons in each animal, including in the SIADL, SIADR, SIAVL, and SIAVR neurons.
• Primary Identifier: Expr2772
• Remark: When expressed in a sup-9(lf);unc-93(gf) mutant, the sup-9::gfp reporter could restore the rubberband Unc phenotype caused by an unc-93(gf) allele, indicating that this reporter fusion protein is functional.
• Subcellular Localization: SUP-9::GFP expression was observed along the surface of body-wall muscle cells, with a punctate stripe pattern characteristic of dense bodies, structures functionally analogous to vertebrate Z-lines, which connect the myofibril lattice to the cell membrane. Additional staining was observed in muscle arms, which project from the muscle body toward neurons to form synapses.

7 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
Longitudinal bands of muscle cells surrounding animal body, with one band running in each quadrant of the body, regulated contraction and relaxation of these muscles cause locomotion.	body wall musculature	body muscle	WBbt:0005813
muscle associated with hermaphrodite vulva.	vulval muscle		WBbt:0005821
neuron with its cell body situated in the head, excluding the pharynx.	head neuron		WBbt:0006751
Receives a few synapses in the ring, has a posteriorly directed process that runs sublaterally	SIAVL	lineage name: ABplpapappa	WBbt:0004985
Receives a few synapses in the ring, has a posteriorly directed process that runs sublaterally	SIAVR	lineage name: ABprpapappa	WBbt:0004983
Receives a few synapses in the ring, has a posteriorly directed process that runs sublaterally	SIADL	lineage name: ABplpapaapa	WBbt:0004989
Receives a few synapses in the ring, has a posteriorly directed process that runs sublaterally	SIADR	lineage name: ABprpapaapa	WBbt:0004987

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00006318	sup-9	F34D6.3	Caenorhabditis elegans

6 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021