WormMine: ExpressionPattern Expr1613

• Pattern: Reproducible beta-galactosidase expression was first observed in 24 cells at the 55-cell (32-AB-cell) stage in embryos carrying the lin-12::lacZ construct; variable staining was observed at the 28-, 46- and 50-cell stages. The positions of the beta-galactosidase-expressing cells suggested that all 24 expressing cells at the 55-cell stage were AB-derived. laser-ablation of AB in the 2-cell embryo eliminated all beta-galactosidase expression at a time corresponding to the wild-type 55-cell (32-AB-cell) stage. In intact transgenic embryos, beta-galactosidase expression persisted through at least one round of cell division. Again, because an engineered beta-galactosidase with a short half-life was used in the construction of the translational fusion, this observation may reflect continued expression of the lin-12 reporter. There were no reporter expression in either ABplaa or ABplpa. However, lin-12 expression was observed in the descendants of both of these cells. lin-12-expressing cells included ABplaaa and ABplpapp.
• Primary Identifier: Expr1613
• Remark: Reporter gene fusion type not specified.

2 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
embryonic cell	ABplaaa		WBbt:0005905
embryonic cell	ABplpapp		WBbt:0006420

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00003001	lin-12	R107.8	Caenorhabditis elegans

1 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The C. elegans life stage spanning 100-290min after first cleavage at 20 Centigrade. Proliferate from 28 cells to 421 cells. Referring to the whole period of gastrulation.		gastrulating embryo Ce	WBls:0000010