| Primary Identifier | Expr15541 | Subcellular Localization | In spermatozoa, both MFP2 and MSD-1 antibodies localize to the pseudopod, matching the known pattern in Ascaris spermatozoa. Within prophase and meiotically dividing spermatocytes, MFP2 and MSD-1 antibodies labelled discrete structures throughout the cytoplasm in a pattern strongly resembling FBs labelled by MSP antibodies. To verify that NSPH-2 and MSD-1 localized to FBs, we co-labelled male germline with anti-MSP and either anti-MSD-1 or anti-NSPH-2 antibodies. Within spermatocytes, the fully overlapping patterns revealed that NSPH-2 and MSD-1 are packaged along with MSP in the FBs. In spermatids, the MSP and MSD-1 were overlapping. In contrast, except in immature spermatids that still retained their FBs, the NSPH-2 and MSP patterns only partially overlapped. In spermatids, the NSPH-2 labelling was notably less robust which may suggest that a change in protein conformation or binding partners is blocking the antigenic site. |
| Definition | Name | Synonym | Primary Identifier |
|---|---|---|---|
| Male haploid germ cell. | sperm | spermatozoa | WBbt:0006798 |
| A cell which gives rise to spermatids by meiosis. | spermatocyte | WBbt:0006799 |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00013956 | nsph-2 | ZK265.3 | Caenorhabditis elegans |
