WormMine: ExpressionPattern Expr2922

• Primary Identifier: Expr2922
• Subcellular Localization: SPD-2 not only associates with centrosomes, but it does so at nearly all of the developmental stages examined. In the hermaphrodite gonad, SPD-2 localizes as discrete perinuclear foci in the mitotic portion of the germline. These foci were also evident during the early stages of oogenesis but were absent in mature oocytes. In contrast, mature sperm retained these SPD-2 foci with each male gamete containing a single dot adjacent to the nucleus. Similarly, in meiotic stage embryos, a single SPD-2-positive dot was observed next to the male pronucleus. In slightly older embryos, one or two perinuclear foci of SPD-2 staining were observed. The intensity of the foci increased with the age of the embryos and at mitosis both spindle poles were brightly stained. As embryos progressed through anaphase and telophase, the intensity of staining gradually diminished. Careful examination of the staining pattern at this stage revealed a diffuse area of SPD-2 material centered on one or two very bright dots. To determine if these central dots corresponded to the position of centrioles, SPD-2 distribution was examined in a strain expressing a GFP-tagged version of the centriole marker SAS-4 and it was found that the SPD-2 and SAS-4-positive dots coincided. SPD-2 probably localizes to both centrioles as well the pericentriolar region. Centrosome staining was also evident in older embryos and SPD-2-positive puncta were seen in young larvae, suggesting that SPD-2 is present at the centrosome throughout the entire life cycle. SPD-2 staining, however, was not restricted to the centrosome. Varying amounts of SPD-2 were also noticed present in the cytoplasm and nuclei of oocytes and embryos.

1 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
organ producing either sperm or ova.	gonad		WBbt:0005175

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00004953	spd-2	F32H2.3	Caenorhabditis elegans

6 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003