| Pattern | Expressed in oocytes, gonad and all cells during in early embryo. | Primary Identifier | Expr2828 |
| Remark | When microtubules were depleted via tba-2 RNAi, centrosomal localization of ZYG-12A,B,C::GFP was lost although the nuclear envelope localization was completely normal. These data are consistent with localization of ZYG-12 to the centrosome arising by direct or indirect binding of ZYG-12 to microtubules. | Subcellular Localization | ZYG-12A,B,C::GFP localized to the nuclear envelope in the syncytial gonad, oocytes, and all nuclei during the development of the early embryo. The tagged ZYG-12 isoforms also localized to the region around the centrosome. This hollow sphere of localization appeared similar to that of the proximal ends of microtubules organized by the centrosome. Labeled microtubules extended in a linear fashion to the cortex, whereas the ZYG-12 labeling was concentrated at the centrosome and did not extend more than a few m away from the centrosome. ZYG-12A::GFP alone localized to only the centrosome and was not detected at the nuclear envelope. Expression of ZYG-12B,C::GFP resulted in localization to both the nuclear envelope and centrosomes. Expression of either ZYG-12B::GFP or ZYG-12C::GFP alone resulted in identical localization as ZYG-12B,C::GFP. |
| Definition | Name | Synonym | Primary Identifier |
|---|---|---|---|
| a female haploid germ cell. | oocyte | ovum | WBbt:0006797 |
| organ producing either sperm or ova. | gonad | WBbt:0005175 |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00006997 | zyg-12 | ZK546.1 | Caenorhabditis elegans |
