| Primary Identifier | Expr9205 | Subcellular Localization | MACO-1 antibody staining was detected in most or all C. elegans neurons and restricted to the neuronal cell body: no staining was observed in dendrites, in the synapse-rich axon bundles that make up the nerve ring, or in the axons comprising the ventral and dorsal cords. To confirm that MACO-1 was absent from synapses, we co- stained worms expressing the synaptic marker SNB-1-GFP in GABAergic neurons (juIs1) with anti-MACO-1 and anti-GFP antibodies: the two markers did not show co-localization. Worms co-expressing juIs1 and psnb-1::maco-1-mcherry showed that transgenic MACO-1-cherry was restricted to the cell body of neurons, overlapping only with the SNB-1-GFP signal in the cell body of GABAergic neurons. Both transgenic and endogenous MACO-1 was also found restricted to neuronal cell bodies at embryonic developmental stages. Co-immunostaining of MACO-1 and YFP revealed partial co-localization between MACO-1 and the ER general marker, YFP- PIS. Within the ER, MACO-1 was further co- localized with a marker restricted to the rough ER, YFP-TRAM, and nuclear envelope, YFP-Emerin. Similar ER localization was seen in embryonic stages. No significant co-localization was observed with the Golgi-specific marker, YFP-MANS; however the cell bodies of C. elegans neurons are small (2 microns), making it difficult to exclude the possibility that there is a low amount of MACO-1 in Golgi. MACO-1 predominantly resides in the ER of neurons, in particular in rough ER and nuclear envelope. |
| Definition | Name | Synonym | Primary Identifier |
|---|---|---|---|
| neuron that uses GABA as a vesicular neurotransmitter. | GABAergic neuron | WBbt:0005190 |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00017066 | maco-1 | D2092.5 | Caenorhabditis elegans |
