WormMine: ExpressionPattern Expr9679

• Pattern: Animals transgenic for madd-4Ap::RFP express RFP in the commissural GABAergic and cholinergic motor neurons. RFP expression is weak in hatchlings and increases dramatically in only the cholinergic commissural motor neurons during the second larval stage, which is coincident with the period of larval muscle arm extension (Dixon and Roy, 2005). This pattern of commissural motor neuron-specific expression persists into adulthood and no other cells or stages express RFP from the madd-4A promoter.
• Primary Identifier: Expr9679
• Reporter Gene: [madd-4Ap::RFP] transcriptional fusion. The madd-4A reporter pPR681(madd-4Ap::RFP) consists of 3.2 kb of sequence upstream of the MADD-4A initiator methionine that drives the expression of red fluorescent protein (RFP).

2 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
neuron that uses GABA as a vesicular neurotransmitter.	GABAergic neuron		WBbt:0005190
neuron that uses vesicular acetylcholine as a neurotransmitter.	cholinergic neuron	ACh neuron	WBbt:0006840

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00009958	madd-4	F53B6.2	Caenorhabditis elegans

5 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027