WormMine: ExpressionPattern Expr2574

• Pattern: The developmental profile of the lin-11::GFP vulval expression in all three lines is nearly identical, although their fluorescence brightness can be ranked nIs96>syIs80>syIs53. The syIs80 and syIs53 animals reveal dynamic changes in the vulval GFP expression. The earliest GFP expression in syIs80 vulval cells is detected in one of the two daughters of the 2 lineage precursors (P5.pp and P7.pa cells). In most cases, GFP fluorescence was detectable only ~1-2 hours before the VPC daughters were beginning to divide. At this stage, expression in P6.p daughters is much weaker and rarely observed. During the Pn.pxx stage, vulval cells begin to reveal brighter GFP fluorescence in both the 1 and 2 lineages. In the 2 lineage, expression is typically seen in only the N and T cells. By the Pn.pxxx stage, lin-11::GFP expression is detected in all 2 lineage progeny. In general, vulA has the lowest level of expression compared with others. syIs53 animals reveal a similar pattern of expression, although the overall fluorescence is considerably reduced. By mid-L4 stage, the GFP fluorescence in syIs53 and syIs80 strains begins to fade, and can not be seen by late-L4 stage. However, in nIs96 animals fluorescence can be detected in young adult animals. Expression is also detected in the uterine lineage cells, VC neurons and a subset of the head and tail neurons. In addition, expression in the B.pap and its descendents was observed in the developing male proctodeum.
• Primary Identifier: Expr2574
• Remark: No detailed description on expression pattern in other life stage or anatomy parts. Reporter gene fusion type not specified.

18 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
neuron with cell body associated with the ventral nerve cord.	ventral cord neuron	ventral cord motoneuron	WBbt:0005300
neuron with its cell body situated in the head, excluding the pharynx.	head neuron		WBbt:0006751
neuron with its cell body situated in the tail, posterior to rectum.	tail neuron		WBbt:0006759
Neuron class of six motoneurons in the ventral cord, which innervate ventral body muscles and the vm2 muscles of the vulva.	VC neuron		WBbt:0005304
	P6.p hermaphrodite		WBbt:0008125
descendent cell of post-embryonic blast cell P5, posterior, in hermaphrodite.	P5.p hermaphrodite		WBbt:0008121
	P7.p hermaphrodite		WBbt:0008129
adult vulval toroid cell, F, formed by fusion of precursors of lineage names P6.papl, P6.papr, P6.ppal and P6.ppar.	vulF		WBbt:0006768
adult vulval toroid cell, E, formed by fusion of precursors of lineage names P6.paal, P6.paar, P6.pppl and P6.pppr.	vulE		WBbt:0006767
cell, anterior descendent of P7.p	P7.pa		WBbt:0006983
post-embryonic cell of pedigree P5.pp	P5.pp		WBbt:0007255
adult vulval toroid cell, C, formed by fusion of precursors of lineage names P5.ppal, P5.ppar, P7.papl and P7.papr.	vulC		WBbt:0006765
adult vulval toroid cell, D, formed by fusion of precursors of lineage names P5.ppp and P7.paa.	vulD		WBbt:0006766
adult vulval toroid cell, B2.	vulB2		WBbt:0006764
adult vulval toroid cell, B1, formed by fusion of precursors of lineage names P5.papa and P7.ppap.	vulB1		WBbt:0006763
adult vulval toroid cell, A, formed by fusion of precursors of lineage names P5.paaa, P5.paap, P7.pppa and P7.pppp	vulA		WBbt:0006762
toroidal epithelial cell that make up the bulk of the uterus, four cells anterior and four cells posterior to the vulva.	uterine toroidal epithelial cell	ut cell	WBbt:0006784
Cell of male proctodeum, post-embryonic pedigree B.pap.	B.pap	lineage name: B.pap	WBbt:0004811

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00003000	lin-11	ZC247.3	Caenorhabditis elegans

2 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038