|
Another strain generated with this plasmid, UL191, shows similar expression to UL256, though the component involving the seam cell expression is weaker and expression of the intestinal nuclei is stronger. Legacy Data: "Bauer PK" "Hope IA"Date 1998-01. |
|
Expr107
|
In adults nuclear-localised expression is observed in the hypodermal seam cells running from the head to the tail and in additional cells of the tail that could be the phasmids. There is some expression in other sites which may be artefactual. Weak and non-localised expression is also occasionally seen in late embryos and young larvae. Young larvae may exhibit strong non-localised staining of the pharynx which may persist to later stages. Expression has also been seen in the posterior intestinal nuclei or non-localised throughout the intestine. Such expression has been attributed to use of incomplete promoters but the upstream region in pUL#PB27 is 2.7kb, which is quite large and includes all but 1kb of the intergenic region. |
|
|
Probably also expressed at postembryonic stages, but actual stages was not mentioned clearly in the paper. |
|
Expr1627
|
MUA-3 also localizes to sites where other striated muscles make adhesive contact and transmit force across the hypodermis. These include the vulval muscles, the anal depressor muscle, and the junctions between anal sphincter muscle and rectal cuticle that cross the intestinal/rectal epithelium. MUA-3 localizes to several sites where non-muscle cells make tight transhypodermal contacts to the cuticle. It is present in thin longitudinal stripes where the touch neurons (ALM and PLM) compress the hypodermis to make close contact with the cuticle, and where the sensory dendrites of the amphid, phasmid, and IL neurons penetrate the hypodermis and cuticle to form externally exposed sensilla. MUA-3 also localizes to the excretory duct and pore cells, both of which contain a cuticle lined lumen. MUA-3 localizes to several sites where nonmuscle cells make tight transhypodermal contacts to the cuticle. It is present in thin longitudinal stripes where the touch neurons (ALM and PLM) compress the hypodermis to make close contact with the cuticle, and where the sensory dendrites of the amphid, phasmid, and IL neurons penetrate the hypodermis and cuticle to form externally exposed sensilla. MUA-3 also localizes to the excretory duct and pore cells, both of which contain a cuticle lined lumen. MUA-3 was not detected at the uterine seam, where the uterus makes indirect IF mediated contact to the cuticle via the seam cells, or in the pharynx, where IF bundles link the basal lamina to the lumenal cuticle. The major site of MUA-3 localization is the hypodermis at the sites of muscle contact. The immunofluorescence coincides with the extent of the body wall muscle bands and shows a repeating pattern of closely spaced circumferential stripes (~0.4 um periodicity) separated by an unstained gap (~0.2 um) in adults. Individual stripes are not uniformly stained, but rather composed of many individual spots and frequently contain a narrow central nonstaining region. Where neuronal processes interpose between hypodermis and basal lamina, MUA-3 is absent, resulting in stereotypical gaps. The MUA-3 localization pattern is virtually identical with those reported for MH4 and p70 (IF proteins) and MH46 (myotactin). During embryogenesis, MUA-3 is observed in 2-fold and older embryos. |
|
