WormMine: AnatomyTerm WBbt:0004762

• Definition: Neuron class of one male-specific sensory neuron associated with hook sensillum, receptor anterior to cloaca in male.
• Name: HOA
• Primary Identifier: WBbt:0004762
• Synonym: lineage name: P10.pppa

1 Children

Definition	Name	Synonym	Primary Identifier
nucleus of pedigree P10.pppa	P10.pppa nucleus		WBbt:0002084

0 Expression Clusters

15 Expression Patterns

• Primary Identifier: Expr15340
• Remark: Promoter 6-1

• Primary Identifier: Expr9260
• Pattern: In the head of both males and hermaphrodites, the expression of the Ptbb-4::YFP transcriptional reporter was observed in many amphid neurons, likely ADL, AFD, ASE, ASG, ASH, ASJ, ASK, ASI, AWA, AWB, and AWC as judged by co-labeling with lipophilic dyes and/or cellular morphology. Other head neurons expressing Ptbb-4::YFP include the cephalic CEP neurons, the outer labial quadrant OLQ neurons, and the mechanosensory FLP neurons. In the midbody and tail, Ptbb-4::YFP expression was found in the PDE postdeirid neurons, PQR (a sensory neuron whose cilium is exposed to the pseudocoelomic fluid), and the phasmid neurons PHA and PHB. Ptbb-4::YFP was also expressed in all 18 ray neurons in the male, consistent with a previous report (Portman and Emmons 2004). Because of abundant ray neuron expression, Ptbb-4::YFP expression in male phasmid and hook neurons could not be reliably confirmed, although expression in these cells is not unlikely. We also observed Ptbb-4::YFP expression in the hermaphrodite HSN motor neurons, the vulval muscles, and several unidentified nonsensory neurons. Expression was also occasionally apparent in the amphid sheath (AMsh) and socket (AMso) glial cells.

• Primary Identifier: Expr9261
• Pattern: In the head, a Ptba-9::YFP transcriptional reporter showed expression in amphid neurons, likely to be ADF, AFD, ASE, ASI, AWA, and AWC, in addition to the OLQ and CEP neurons, the anterior and posterior deirid neurons ADE and PDE, the RIG interneuron, and several additional unidentified nonsensory neurons. In the posterior of both sexes, this reporter was expressed in two tail interneurons, likely DVA, DVB, and/or DVC, as well as the sensory neuron PQR. In the male tail, Ptba-9::mCherry was expressed in the hook HOA and the ray RnA neurons as judged by morphol- ogy, position, and lack of overlap with the RnB marker Ppkd-2::GFP. In the head, a Ptba-9::YFP transcriptional reporter showed expression in amphid neurons, likely to be ADF, AFD, ASE, ASI, AWA, and AWC, in addition to the OLQ and CEP neurons, the anterior and posterior deirid neurons ADE and PDE, the RIG interneuron, and several additional unidentified nonsensory neurons. In the posterior of both sexes, this reporter was expressed in two tail interneurons, likely DVA, DVB, and/or DVC, as well as the sensory neuron PQR. In the male tail, Ptba-9::mCherry was expressed in the hook HOA and the ray RnA neurons as judged by morphology, position, and lack of overlap with the RnB marker Ppkd-2::GFP.

• Primary Identifier: Expr1628
• Pattern: In wild-type animals, the mab-21::gfp fluorescent signal was first observed in embryos at the beginning of gastrulation. The expressing cells were dorsal hyp 7 cells before their fusion. From this stage on, the signal could be detected in other larval and adult animals in tissues including hypodermal cells, a group of neuronal cells near the pharyngeal bulbs, neurons along the ventral nerve cord, some body wall muscles and the ray cells. Neuronal cells around the anal ganglia region identified as HOA, HOB in the males as well as PLM, PQR, and PVC. In addition to two unidentified anterior neurons, AVM and VA3 were also confirmed to express mab-21::gfp. The mab-21::gfp fusion transgene was expressed in neuron A of all rays, neuron B of rays 3 and 6 and more importantly, the structural cell of only ray 6.

• Primary Identifier: Expr950
• Pattern: Early in development, 1497pro-mab-3::gfp was expressed in head neurons of late embryos and L1 larvae of both sexes. Later in larval development, starting in L3, it was expressed in the lateral hypodermis (seam) in both sexes, and male-specifically in the nervous system, both in the head and tail, including the sensory ray neuroblasts R1-R9 in the tail. Initially there is some expression in the intestine of both sexes, but expression becomes male-specific by the adult stage, when vitellogenin transcription begins in hermaphrodites. Early in development, pro-mab-3::gfp was expressed in head neurons of late embryos and L1 larvae of both sexes. Later in larval development, starting in L3, it was expressed in the lateral hypodermis (seam) in both sexes, and male-specifically in the nervous system, both in the head and tail, including the sensory ray neuroblasts R1-R9 in the tail. Initially there is some expression in the intestine of both sexes, but expression becomes male-specific by the adult stage, when vitellogenin transcription begins in hermaphrodites.

• Primary Identifier: Expr9999
• Pattern: A 2.8 kb promoter of cysl-1 drove GFP expression mainly in the nervous system of adult hermaphrodites. The cysl-1::GFP expression pattern was similar for the transcriptional and translational reporters. GFP was observed in subsets of pharyngeal neurons, amphid sensory neurons and tail neurons, starting from late embryonic stages and persisting into adults. We identified GFP-positive cells as the AVM sensory neuron, the BDU interneurons, and the pharyngeal I1 interneurons and M2 motor neurons, based on their characteristic processes and nuclear positions. GFP in body wall muscles, hypoderm, and intestine was present in larvae but only weakly detectable in adult animals.

• Primary Identifier: Expr12313
• Pattern: In these transgenic males, UNC-7 expression was found, in addition to the sex muscles (Expr12312), in several mating circuit neurons (SPC, PCA, PCB, HOA, and unidentified ray neurons). Similar to previous studies (Starich et al., 2009; Yeh et al., 2009; Kawano et al., 2011), this reporter also expresses in head ganglion neurons, several ventral chord neurons, and tail neurons of the hermaphrodite, in addition to few unreported pharyngeal neurons (I5, M2, I4, M3, and I1).

• Primary Identifier: Expr10584
• Pattern: The prig-6RIG-6::GFP construct drives expression of all RIG-6 isoforms in fusion with GFP, under their endogenous promoter. rig-6 expression is detected during embryogenesis and is maintained throughout adulthood. rig-6 is expressed in I1 and I3 pharyngeal interneurons, NSM motor neurons and other unidentified head neurons. RIG-6::GFP is also localized in the cell bodies and axons of different subtypes of VNC motorneurons, in the HSN and the CAN neurons. Neuronal expression of rig-6 also includes the ALM and PLM touch receptor neurons. Non-neuronal cells expressing rig-6 include somatic muscles and the hypodermis. We also detected rig-6 expression in the developing and adult EC. The prig-6RIG-6::GFP construct drives expression of all RIG-6 isoforms in fusion with GFP, under their endogenous promoter. rig-6 expression is detected during embryogenesis and is maintained throughout adulthood. rig-6 is expressed in I1 and I3 pharyngeal interneurons, NSM motor neurons and other unidentified head neurons. RIG-6::GFP is also localized in the cell bodies and axons of different subtypes of VNC motorneurons, in the HSN and the CAN neurons. Neuronal expression of rig-6 also includes the ALM and PLM touch receptor neurons. Non-neuronal cells expressing rig-6 include somatic muscles and the hypodermis. We also detected rig-6 expression in the developing and adult EC.

• Primary Identifier: Expr12408
• Pattern: With a construct including 8 kb upstream of the first exon and the entire unc-7 genomic region fused to YFP, UNC-7 expression was found, in addition to the sex muscles reported in Expr12407, in several mating circuit neurons (SPC, PCA, PCB, HOA, HOB, and unidentified ray neurons). Similar to previous studies (Starich et al., 2009; Yeh et al., 2009; Kawano et al., 2011), this reporter also expresses, in head ganglion neurons, several ventral chord neurons, and tail neurons of the hermaphrodite, in addition to few unreported pharyngeal neurons (I5, M2, I4, M3, and I1).

• Primary Identifier: Expr12405
• Pattern: Pavr-14:YFP was expressed in several head, ventral chord, dorsal chord, and tail neurons. avr-14 expression was found in HOA.

• Primary Identifier: Expr14473
• Pattern: The ttll-11a promoter drove GFP expression in a distinct and non-overlapping set of ciliated sensory neurons in the head, including the IL1s, outer labial quadrant neurons (OLQs), cephalic neurons (CEPs), and amphids, but not in the extracellular vesicle-releasing neurons EVNs. In the male tail, expression was seen in the hook A type neuron (HOA), ray type A neurons (RnAs), and phasmids but not the HOB and RnB neurons. Expression was also seen in phasmid neurons in the hermaphrodite tail.

• Primary Identifier: Expr12033
• Pattern: The cilium structural gene osm-6 is expressed in both HOA (P10.pppa) and HOB (P10.ppap).

• Primary Identifier: Expr11947
• Pattern: A pkd-2-gfp fusion vector, that partially rescued the response phenotype of pkd-2-mutant males, was expressed in the CEM neurons, HOA, HOB, and sensory ray neurons and in the rays. Developmental regulation of expression was seen, appearing at L4 and peaking in adult males.

• Primary Identifier: Expr11948
• Subcellular Localization: A polyclonal anti-polycystin-2 peptide antibody confirmed expression of polycystin-2 in the cytoplasm of the cell bodies and neuronal processes of 4 male-specific cephalic neurons in the head. In the male tail, staining was seen in the cell bodies of both sets of 9 neurons that innervate the sensory rays on each side of the tail as well as in the HOA and HOB neurons that innervate the hook, used to detect the hermaphrodite vulva.

• Primary Identifier: Expr12406
• Pattern: Pdop-2:GFP expression was observed in HOA neuron at the adult male tail.

0 Life Stages

2 Parents

Definition	Name	Synonym	Primary Identifier
Hook sensory neurons.	HO neuron		WBbt:0005177
post-embryonic cell of pedigree P10.ppp	P10.ppp		WBbt:0007223