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Expr9584
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hlh-14 is first expressed at the AB64-cell stage. hlh-14 expression appears to persist longer in posterior parts of the ABalppp/praaa lineage than in anterior parts. In the ASE branch, located at the posterior part of the lineage, hlh-14 expression is maintained throughout the lineage until shortly before the terminal division of the ASE neurons. In addition to its expression in the ASE lineage, it was observed by 4Dlineage analysis that the hlh-14 fosmid reporter is expressed in several other neuronal lineages. In some instances this expression is bilaterally symmetric, such as in the neuroblasts that give rise to the L/R bilaterally symmetric PLM/ALN neuronal pairs, PVQ/HSN/ PHB neuron pairs and the ALM/BDU neuron pairs. Surprisingly, in other instances this expression is L/R asymmetric, such as in neuroblasts of the C lineage. We observe asymmetric expression in two asymmetrically generated neuroblasts, the Caapa neuroblast that gives rise to the DVC neuron and a cell death, and the Caappv cell, which differentiates into the neuron PVR. The bilateral homologs of these hlh-14-expressing neuroblasts are non-neuronal hypodermal cells. Intriguingly, we also observe expression in the bilaterally symmetric neuroblasts that produce a pair of pharyngeal interneurons known as I1L/I1R. Finally, we observe later expression of hlh-14 in currently unidentified lineages, at a time in which all terminally differentiated cells present at hatching are already formed. |
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