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Expr1164
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Transgenic animals that carry this construct show GFP expression in a variety of tissue types. GFP expression is observed in the intestine, and the posterior cells express GFP more intensely than the remaining intestinal cells. Other cells include the rectal epithelial cells, the pharynx, the somatic gonad, and vulval hypodermal cells. In addition, the IP3 receptor is expressed in hypodermal cells of the tail, rectum, and head. Pharyngeal expression is restricted to the muscles of the metacorpus, isthmus, and the anterior portion of the terminal bulb (m4, m5, and m6). This construct was only expressed in neurons LUA and PDA. GFP is expressed in the gonad sheath cells, spermatheca, spermathecal valve, and uterine sheath cells. GFP is expressed in the vulval hypodermal cells. |
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Expr1816
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All three reporter constructs were expressed in all major contractile tissues, starting during embryogenesis and continuing until adulthood. Fluorescence was particularly pronounced in striated muscle (body wall muscles used for locomotion), non-striated muscle (pharyngeal muscles used for pharyngeal pumping, vulval and uterine muscles used for egg laying, the sphincter muscle and anal depressor used for defecation), and in myoepithelial cells (gonadal sheath cells used for ovulation). Furthermore, all three constructs showed expression in the intestine. Some cells expressed CeSERCAb::GFP but not CeSERCAa::GFP. These include the somatic cells of the spermatheca and the excretory canal and the uterine sheath cells. No cells were found that expressed CeSERCAa::GFP but not CeSERCAb::GFP. |
Fluorescence from Psca-1::GFP was evenly distributed over the cytosol. However, both CeSERCAa::GFP and CeSERCAb::GFP showed specific subcellular localization. Both fusion proteins were found in a tubular meshwork that was most distinct in the body wall muscle cells. This staining was continuous with staining at or near the dense bodies, structures functionally analogous to vertebrate Z-lines. In body wall muscle cells and other cells, staining was also often localized in internal vesicles and membrane-like structures, including the nuclear envelope. |
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[CEH-63::gfp] translational fusion. |
Expr9877
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CEH-63 expression was observed in DVC and the uterus. The GFP signal was localized to the cell nuclei, presumably due to the DNA binding activity of the transcription factor taking the fusion protein to the nucleus. The nuclear localization allowed identification of the uterine cells expressing the CEH-63 C-terminal GFP fusion as ut2 and ut3. This identification was made in young adults after uterine development was complete. Although no extra expression was observed for the N-terminal fusion, for the C-terminal fusion there was also reliable expression in 6-10 nuclei in the head region and at a similar level to that in DVC. There was also weak but frequent expression of the C-terminal fusion in two other unidentified nuclei, posterior to DVC, in the hermaphrodite tail. CEH-63 expression was observed in DVC and the uterus. The GFP signal was localized to the cell nuclei, presumably due to the DNA binding activity of the transcription factor taking the fusion protein to the nucleus. The nuclear localization allowed identification of the uterine cells expressing the CEH-63 C-terminal GFP fusion as ut2 and ut3. This identification was made in young adults after uterine development was complete. Although no extra expression was observed for the N-terminal fusion, for the C-terminal fusion there was also reliable expression in 6-10 nuclei in the head region and at a similar level to that in DVC. There was also weak but frequent expression of the C-terminal fusion in two other unidentified nuclei, posterior to DVC, in the hermaphrodite tail. |
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