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Expr14915
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vglu-2::gfp is dynamically expressed in a restricted set of distinct cell types. Within the nervous system, vglu-2::gfp is exclusively expressed in a single interneuron class, AIA, where it localizes to vesicular structures in the soma, but not along the axon, suggesting that VGLU-2 may not be involved in synaptic transport of glutamate. The expression in AIA can be observed throughout alllarval and adult stages in either hermaphrodite or male animals. Outside the nervous system, VGLU-2 is expressed in collagen-producing skin cells (syncytial epidermis and in uterine tissue) where it most prominently localizes to early endosomes, and to a lesser degree apical clathrin-coated pits, TGN, and late endosomes. On early endosomes VGLU-2 colocalized most strongly with the recycling promoting factor SNX-1, a retromer component. |
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Expr15046
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Co-expression of scyl-1 and slo-2 was observed in many ventral cord motor neurons. However, most other neurons expressing slo-2 (e.g. head and tail neurons) did not appear to express scyl-1. In addition, scyl-1 expression was detected in some cells that did not express slo-2, including the excretory cell, spermatheca, uterine ventral cells, and intestinal cells. |
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Reporter gene fusion type not specified. |
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Expr1325
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The GFP::TLN-1 reporter showed prominent expression in all differentiating vulval and uterine cells. In addition to the basal and lateral plasma membrane- associated signal, GFP::TLN-1 expression was also observed in the cytoplasm of the vulval cells of wild-type L3 larvae. lin-26 transcripts were first weakly detected by in situ hybridization in the germline precursors (P3, P4 and Z2/Z3) until the 50-cell stage and occasionally in their sisters. After the 100-cell stage, lin-26 transcripts and the beta-galactosidase encoded by the lin-26::lacZ construct were detected in hypodermal precursors, then in differentiating hypodermal and support cells. The only difference between in situ hybridization and antibody staining is that lin-26 transcripts could not be detected in body hypodermal cells after the 350-cell stage nor in any cell after the comma stage, whereas LIN-26 protein can be detected until the end of embryogenesis in all hypodermal cells and in all support cells. |
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Expr3686
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Transgenic animals containing the fos-1a::YFP-TX and -TL reporters showed the same expression pattern at all times examined, and fos-1a::YFP-TL protein localized to the nucleus. fos-1a::YFP-TL also rescued the AC-invasion defect when expressed in fos-1(ar105) mutants. fos-1a was expressed at the highest level in the AC and at lower levels in neighboring somatic gonad cells during AC invasion. No expression of fos-1a was detected in vulval cells. In contrast, both the fos-1b::CFP-TX and -TL reporters directed expression in vulval cells and the AC. fos-1b::CFP-TL also drove expression in neighboring uterine cells, whereas fos-1b::CFP-TX did not, suggesting the presence of a downstream uterine enhancer element in fos-1b::CFP-TL. Unlike fos-1a expression, levels of fos-1b::CFP-TL protein were not greater in the AC compared to neighboring cells. Moreover, whereas the fos-1a reporters drove expression almost exclusively in the somatic gonad cells, the fos-1b reporters directed expression in nearly all cells at the L3 stage. |
fos-1a::YFP-TL protein localized to the nucleus |
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Expr9292
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The Y45F10C.2 promoter was most active in the uterine epithelium. |
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Expr13968
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WSP-1/N-WASP was present at the Anchor Cell invasive membrane before and after invasion, although it was also visible at cell junctions elsewhere in the vulval and uterine cells. Spots of WSP-1/N-WASP on the invasive membrane colocalized with spots of WVE-1/WAVE. |
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Expr15011
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Both reporters, gfp::egl-43L and egl-43::gfp, were expressed in the invading AC and in the surrounding ventral and dorsal uterine (VU and DU) cells. We found no obvious difference in the expression pattern of the two egl-43 reporters, suggesting that the long egl-43L isoform accounts for most of the expression observed. |
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Expr11523
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Endogenous nuclear EGL-4 was detected in the nucleus of uterine epithelial cells. |
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Expr13257
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Wild-type PAT-3::GFP was expressed on the lateral membranes of the vulval cells and along the basal laminae that separate the vulval cells from the uterus. Since PAT-3::GFP was also expressed in the adjacent uterine cells, both tissues are likely to contribute to the strong signal along the basal laminae. PAT-3::GFP localization on the lateral membranes of the vulval cells was most prominent at the onset of vulval invagination in mid to late L3 larvae. |
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Expr13258
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The GFP::TLN-1 reporter showed prominent expression in all differentiating vulval and uterine cells. In addition to the basal and lateral plasma membrane- associated signal, GFP::TLN-1 expression was also observed in the cytoplasm of the vulval cells of wild-type L3 larvae. |
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Expr14950
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In non-dauer animals, expression was only observed in the uterine and spermathecal cells of early-mid-L4 to adults. ets-10p::gfp expression is undetectable under dissecting scope in embryos to early-L4. |
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Expr16206
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zh118[frt::gfp::mcm-7] animals exhibited GFP::MCM-7 expression in the nuclei of proliferating cells, including many uterine cells and the VPCs. |
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Expr16209
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The adjacent uterine cells showed predominantly cytoplasmic CDT-1::GFP expression. |
