WormMine

WS295

Intermine data mining platform for C. elegans and related nematodes

Expression Pattern :

Pattern  Developing embryos was found carrying ngn-1::gfp and NGN-1::GFP protein was present in the MI mother cell. By contrast, NGN-1::GFP was not detectable in the e3D mother cell, indicating that expression of ngn-1 is left-right asymmetric within this asymmetric cell lineage. Primary Identifier  Expr9152
Remark  Picture: Fig. 4A to 4C. Reporter Gene  [ngn-1p::ngn-1-gfp] translational fusion. A translational ngn-1::gfp fusion gene was created by an in-frame insertion of a gfp-coding sequence into the 3' end of the ngn-1-coding sequence flanked by the 12.4 kb 5' upstream and 1.5 kb 3 downstream genomic sequence. Authors integrated this ngn-1::gfp transgene into the C. elegans genome and found that the ngn-1::gfp transgene rescued the MI transformation of ngn-1(n1921) mutants (see Table S1A in the supplementary material), indicating that this ngn-1::gfp is functional.

1 Anatomy Terms

Definition Name Synonym Primary Identifier
embryonic cell ABaraappaa   WBbt:0006636

1 Genes

WormBase Gene ID Gene Name Sequence Name Organism
WBGene00003595 ngn-1 Y69A2AR.29 Caenorhabditis elegans

0 Life Stages