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Expr12734
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EFF-1 was first detected at the bean stage within individual puncta in the dorsal and ventral hypodermal cells before fusion. Subsequently, EFF-1 appeared as punctate staining in the cytoplasm of hyp6 and hyp7 precursor cells at the comma stage. Following embryonic fusions, EFF-1 remained vesicular, and the number of EFF-1 puncta increased when cell fusion was nearly completed. EFF-1 puncta showed minor co-localization with apical cell junctions detected by anti-DLG-1 antibody. EFF-1 puncta were aligned along longitudinal lines lying parallel to the seam cells. This arrangement might be dictated by the organization of the cytoskeleton in the syncytial hypodermal cells, where actin, intermediate filaments, and microtubules form bundles that run parallel to the seam cells. Thus, EFF-1 is expressed in puncta at the onset, during, and after hypodermal cell fusion in developing embryos. |
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