1 Genes
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00012261 | lpr-3 | W04G3.8 | Caenorhabditis elegans |
| Primary Identifier | Expr14486 | Subcellular Localization | ssSfGFP:: LPR-3 fusion protein accumulated in apical extracellular compartments, including the extraembryonic space and the excretory duct/pore, rectal, and vulval lumens. ssSfGFP::LPR-3 also associated with the larval cuticle and accumulated between the old and new cuticles before and during molting. However, LPR-3 was not associated with the outer surface of old cuticle or with shed cuticle after molting, and it completely disappeared in adults. We infer that LPR-3 is present within a juvenile glycocalyx layer, and that the connection between this layer and the mature cuticle is severed during molting. In the developing vulva tube, where resolution is highest and the glycocalyx is 2-3 μm thick (Gill et al. 2016), ssSfGFP:: LPR-3 localized specifically to a narrow region along the apical membrane. ssSfGFP:: LPR-3 localized apically to a PH::mcherry membrane marker, confirming that it is extracellular. ssSfGFP::LPR-3 localized slightly more membrane-proximal than the ZP protein LET-653, indicating that it is present in one of the inner layers of the glycocalyx. |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00012261 | lpr-3 | W04G3.8 | Caenorhabditis elegans |
