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Expr1837
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Expression of eff-1p::gfp was silent through the first third of embryogenesis, first appearing about 230 min after first cleavage in a subset of epidermal precursor cells. Over the next 3 hr, these and additional fluorescent cells were observed to migrate over the ventral and dorsal surfaces of the embryo, and the majority of GFP-positive cells fused to form the hyp6 and hyp7 syncytia. As elongation progressed, GFP was also expressed in a pair of cells that fused to form the binucleate "tail spike" . After hatching, eff-1p::gfp expression persisted in large epidermal syncytia through adulthood. Mononucleated epidermal cells-including the seam cells and the VPCs-remained nonfluorescent until shortly before undergoing larval fusion events. More specifically, GFP was seen in (1) nonstem daughters of the seam cells shortly before they fused into hyp7; (2) vulval cells invaginating to form toroids during morphogenesis; and (3) the rays and fan of the adult male tail. Expression was also seen in nonepidermal organs known to contain syncytia, including the pharynx and uterus. Interestingly, a few cells that express eff-1p::gfp have never been observed to fuse, such as some ventral epidermal precursors in the embryo and several neurons. |
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Three methods, lacZ, gfp, antibody staining results all mixed together. Lots of unextracted cell objects buried in pattern text. |
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Expr841
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PAL-1 produced from zygotic transcripts is seen initially in C and D lineage cells that also expressed maternally derived PAL-1. As gastrulation begins, expression is seen in only Ca and Cp and then in their daughters, of which 2 are hypodermoblasts (Caa and Cpa) and 2 are myoblasts (Cap and Cpp). The GFP reporter is first detected at the late 2C-cell stage and then more strongly in the 4 daughters. At about 100 cells, expression is also detected in the 2 D-lineage myoblasts. Thereafter, PAL-1 continues to be detected in all C and D descendants until the end of gastrulation at about 350 cells. At about 180 cells (midgastrulation), the C hypodermal precursors, which express more strongly than the muscle precursors, form a characteristic double row on each side of the dorsal midline in the posterior. Thereafter, PAL-1 decreases in these cells and is no longer detectable with antibody after 350 500 cells. At about 250 cells, expression is detected in two AB cells that border the posterior left edge of the mesectodermal cell layer that is closing the ventral gastrulation cleft (ABplpappp and ABplppppp) and slightly later in the right homolog of one of them (ABprppppp). The daughters and granddaughters of these cells, generated after the cleft closes, continue to express strongly along the ventral midline until about the time of hatching. Beginning at about 360 cells, as morphogenesis begins, weak transient expression is detected in the posterior ectodermal P cells and occasionally in posterior V cells as both groups move ventrally. During this period the V cells become the lateral seam cells, and the P cells undergo their terminal embryonic divisions as they complete hypodermal enclosure of the embryo. Meanwhile, in the interior, pal-1 expression, detectable both with antibody and with reporter constructs, appears at about 350 cells in 2 Ea descendents near the middle of the gut primordium (the int5 pair) and in 2 anteriorly located MS descendants which migrate to the posterior and become the mesoblast M and the right intestinal muscle (mu intR). During early morphogenesis as the embryo develops through the comma stage and begins to elongate, all the pal-1-expressing cells (approximately 50) are located in the posterior ventral region, except for the 2 midgut cells which lie more dorsally. The descendants of ABpl/rppppp, as well as mu intR, move into the elongating tail and participate in formation of the rectal and associated intestinal muscles, as well as the ventral tail hypodermis. Expression diminishes during elongation and by hatching is detectable only in the 2 gut cells, M, mu intR, and 10 cells descended from ABpl/rppppp. |
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Other strain-- UL693 |
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Expr138
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Diffuse expression of intestinal precursor cells at various embryonic stages. Diffuse intestinal expression in an L2 larva. Diffuse intestinal expression is observed from the embryonic lima bean stage through elongation and into early larval stages. In adult stages expression is seen in components of the intestino-rectal valve. Diffuse expression is also seen in the hypodermis of the tail spike. Expression is seen in components of the adult intestino-rectal valve and some diffuse expression in the tail spike. |
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Expr1853
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During larval development, tlp-1::gfp expressed in the posterior intestinal cells, several neuronal nuclei in the head, the tail tip cells in hermaphrodites and males and in descendants of the T cell lineage. Weak GFP expression observed in the T cell in 6% (n=124) of animals. After the division of the T cell, stronger GFP expression was observed in the posterior T cell daughter, T.p. but not in T.a. More specifically, of the animals that showed GFP expression in the T cell lineage (57/150), 88% of the animals examined showed GFP expression in T.p alone and 12% showed expression in T.a and T.p. GFP expression was also observed in the posterior T.ap daughter cell, T.app but not in T.ap or its anterior daughter cell T.apa. Specifically, all of the animals that showed expression in the T.ap lineage (11/56) showed GFP expression in T.app alone. No expression observed in T.apa in any animals. tlp-1::gfp expression begins to be barely detectable at the beginning of gastrulation at about 100 minutes of embryonic development. Shortly after the gastrulation begins, the level of expression increases and is detectable in the nuclei of most embryonic cells. This pattern appears to persist through gastrulation. However, at about 260 minutes, expression in the anterior of the embryo fades and expression in the posterior of the embryo persists and gets stronger. At the 1.5-fold stage, at about 400 minutes, expression was observed in posterior nuclei and this pattern continues throughout the rest of development. |
nuclei |
