| Primary Identifier | Expr1191 | Remark | Deconvolution microscopy to generate high resolution images of the nuclei of two-cell embryos stained with mAb 6C4 and DAPI. The relative time of each nucleus in the cell cycle was inferred by comparing the staining patterns of the AB and P1 blastomeres; the AB blastomere division occurs before that of the P1 blastomere. The morphology of DAPI staining and presence of a nuclear membrane were used as additional landmarks of cell cycle progression. No other cellular expression pattern described. |
| Subcellular Localization | Interphase nuclei had no detectable mAb 6C4 reactivity. Nuclei that are in the earliest stages of chromosome condensation do not stain with mAb 6C4. Staining with mAb 6C4 was first observed in prophase nuclei that contain condensed chromosomes. At this stage, mAb 6C4 staining was observed as dots distributed throughout the nucleus; many of these dots colocalize with the chromosomes. These chromosome-associated dots are widely dispersed along the entire length of each chromosome. Later in prophase, mAb 6C4 stained structures located on opposite sides of each chromosome. In metaphase, when the chromosomes are aligned at the equatorial plane, chromosomes were oriented with each mAb 6C4 stained structure facing one centrosome. At anaphase, sister chromatids separated with the mAb 6C4 stained side on the poleward face of each sister chromatid. No mAb 6C4 staining was detectable by late anaphase. These results show that there is dynamic localization of the mAb 6C4 antigen to chromosomes throughout mitosis. mAb 6C4 Staining during meiosis During meiosis, C. elegans chromosomes lack a trilaminar kinetochore structure, and attachment to the meiotic spindle is mediated through the chromosome ends. No mAb 6C4 nuclear staining was detected in the mitotic region of the gonad of the hermaphrodite nor in oocytes arrested at diakinesis of prophase I. After fertilization, embryos exit prophase I arrest and undergo two consecutive rounds of division to form two polar bodies. Staining with mAb 6C4 was detected on meiotic chromosomes after fertilization and before first olar body formation. The mAb 6C4 staining pattern was observed as a halo surrounding the meiotic chromosomes. |
| Definition | Name | Synonym | Primary Identifier |
|---|---|---|---|
| Embryonic founder cell | AB | AB blastomere | WBbt:0004015 |
| embryonic founder cell | Psub1 | lineage name: P0.p | WBbt:0006874 |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00001829 | hcp-1 | ZK1055.1 | Caenorhabditis elegans |
| Remark | Definition | Other Name | Public Name | Primary Identifier |
|---|---|---|---|---|
| The C. elegans life stage spanning 0-20min after first cleavage at 20 Centigrade. Contains 2 cells. | 2-cell embryo Ce | WBls:0000007 |
