1 Genes
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00011480 | enpl-1 | T05E11.3 | Caenorhabditis elegans |
| Primary Identifier | Expr15293 | Subcellular Localization | Confocal analysis of ENPL-1::sfGFP worms co-expressing the mCherry tagged rough endoplasmic reticulum (ER) specific marker, SP12(spcs-1) indicated that, as expected, ENPL-1 localized to the ER. Mammalian ENPL-1 homologs are found in non-ER locations in addition to the ER localization (Frasson et al., 2009; Patel et al., 2013). To ask whether this was the case for the worm ENPL-1, we used retrograde back filling of DiI into amphid neurons (Tong and Bürglin, 2010). We found that ENPL-1::sfGFP was present in the cell bodies of these neurons, where the rough ER and Golgi are found (Rolls et al., 2002), as well as in axons and dendrites, where there is no rough ER and Golgi. To further characterize the subcellular distribution of ENPL-1::sfGFP, we performed the differential ultracentrifugation followed by western blotting, to separate and analyze membrane and cytoplasmic fractions. We discovered that the ENPL-1::sfGFP is present in both the membrane and cytosolic fractions. Our analysis indicated that the ENPL-1 is found mainly in the ER lumen, however a fraction of the protein is present outside of the ER. |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00011480 | enpl-1 | T05E11.3 | Caenorhabditis elegans |
