1 Genes
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00001852 | hil-1 | C30G7.1 | Caenorhabditis elegans |
| Primary Identifier | Expr1927 | Remark | C30G7.1 = H1.X Type: mammalian cell transfection. For the expression of H1.X::GFP in human HeLa cells, the H1.X cDNA was amplified with the primers ESMG73 5 -GGGGTACCACCATGGTGACCACTTCGCTCATCCACATGG-3 and MJ12 5 -CGGGATCCCGACAATAGCTCTTTCTGGTTCCGG-3 from the cDNA clone yk480h7 and cloned with KpnI and BamHI into the vector pEGFP-N1 (Clontech). The resulting plasmid expresses H1.X as an unfused protein from the CMV promotor and was used for the transfection of HeLa cells with lipofectamine (GibcoBRL). |
| Subcellular Localization | In HeLa cells, H1.X::GFP does not appear in the cytoplasm but is a strictly nuclear protein. When H1.X::GFP-expressing HeLa cells were fixed and Triton X-100 extracted, the GFP fluorescence of the nucleoplasm was strongly reduced and the nucleoli appeared brightly fluorescent. These results show that H1.X::GFP is a highly soluble protein in the nucleoplasm of HeLa cells and that it specifically binds to the nucleolus, like it does in C. elegans. In fixed as well as living HeLa cells H1.X::GFP is additionally localized in small spot-like structures, which may represent further nuclear bodies. |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00001852 | hil-1 | C30G7.1 | Caenorhabditis elegans |
