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Expr14587
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The integrated smoc-1 transcriptional reporter showed strong GFP expression. GFP was first detectable in several cells located in the anterior of bean stage embryos. In the developing larvae, GFP is expressed in cells of the pharynx, the intestine and the posterior hypodermis. Pharyngeal cells expressing smoc-1p::gfp include the epithelial cells e2, the marginal cells mc1 and mc2, the M4 neuron, and all six of the pharyngeal/intestinal valve cells. Cells of the posterior hypodermis expressing smoc-1p::gfp include hyp8, hyp9, hyp10, and hyp11. Expression in these tissues persisted from the L1 larval stage through adulthood. We noted that while all transgenic animals showed GFP expression in the pharynx and the posterior hypodermis, a small fraction of animals (~8%) did not exhibit GFP expression in all or some of the intestinal cells. We observed no GFP expression in any other tissues, including the nerve cord, body wall muscles (BWMs), or the M lineage. |
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No detailed description on postembryonic expression pattern. |
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Expr1419
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PHA-4 was observed in the nuclei of cells destined to form the digestive tract. At the 4E stage, weak expression is detected in 8 MS great-granddaughters and 10 ABa descendants (ABaraaaa/p, ABaraapa/p, ABarapaa/p, ABalpaaa/p, ABalpapa/p) that each produce pharyngeal cells, as well as nonpharyngeal cells. Faint expression was also detected in the four midgut precursors. Brighter staining is observed in 6 to 8 MS-derived and 16 to 18 ABa-derived cells after the next cell division (MSaaaa/p, MSaapa/p, MSpaaa/p, MSpapa/p). Soon thereafter, at the 8 to 12E stage, PHA-4 is first observed in the rectal precursors. Expression is maintained in all pharyngeal, midgut, and rectal cells throughout embryogenesis. |
nuclei |
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Expr12914
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EFN-4-GFP is detectable in intestinal cells prior to int5 intercalation. EFN-4-GFP is not detectable in intestinal cells at later stages, but is abundant in a subset of pharyngeal and valve cells anterior to the intestine. As expected for a secreted protein, EFN- 4-GFP accumulates at high levels in all visible extracellular spaces within the embryo. |
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Expr16176
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As for IRK-1, IRK-2 is also expected by be targeted to the plasma membrane. The most notable cells labeled by irk-2 promoter expression were neurons of the anterior ganglia, the pharyngeal intestinal valve cell, and the posterior intestine, where again clear basolateral plasma membrane targeting can be observed. In adults, the spermatheca and the uterus were prominently labeled, and the IRK-2 fusion protein resided in the plasma membrane. It is interesting that irk gene expression was notably absent in muscle and hypodermal cells. |
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