Reporter gene fusion not specified |
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Expr16549
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Our transcriptional reporter lines revealed that Cel-nhr-1 is broadly expressed in multiple somatic tissues at varying intensities. We found high expression levels in the entire hypodermis and body wall musculature from head to tail, as well as in the majority of the worm's pharyngeal muscles. The latter includes pm3-5, the cells which make up most of the muscular portions of the procorpus, metacorpus, and isthmus. Relatively lower expression levels were found in the stomatal tissues (aa, ap, e1, e3, pm1, and pm2), the terminal bulb of the pharynx (pm6-8), the intestine, and some ventral aspects of the somatic gonad. |
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Reporter gene fusion not specified |
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Expr16550
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Furthermore, animals carrying the translational reporter express a fluorescently labeled version of NHR-1 that is restricted to the nuclei of the cells in which Cel-nhr-1 is expressed. Specifically, using these localization patterns, we were able to identify the nuclei of the stomatal and pharyngeal muscle cells for which we already have seen expression signals in the transcriptional reporter line. These results confirm the presence of Cel-NHR-1 protein in exactly those cells that secrete the posterior buccal capsule. Additionally, we found Cel-NHR-1 in the nuclei of multiple nerve-ring neurons, possibly including amphid-associated neurons or glia cells. |
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