WormMine: ExpressionPattern Expr16524

• Pattern: Consistent with previous publications, GFP::NRDE-3 (bombardment) localizes to the nuclei of somatic cells beginning around 100-cell stage of embryogenesis. No expression can be detected in germ cells or early embryos. In contrast, GFP::NRDE-3 (CRISPR) [Expr16525] is visible in nuclei beginning in late pachytene of the germline and continues to be expressed in oocytes and in all stages of embryogenesis. This discrepancy in expression between the transgenic strain constructed by bombardment and the CRISPR strain suggests that GFP::NRDE-3 (bombardment) may be silenced in the germline and early embryos.
• Primary Identifier: Expr16524

1 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
Nucleated cell which has a diploid set of chromosomes.	somatic cell	FMA:72300	WBbt:0008378

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00019862	nrde-3	R04A9.2	Caenorhabditis elegans

4 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021
	The C. elegans life stage spanning 350-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The stage that embryo starts elongation until elongation is over.		elongating embryo Ce	WBls:0000015
	The C. elegans life stage spanning 290-350min after first cleavage at 20 Centigrade. Proliferate from 421 cells to 560 cells. The stage when embryo just finished gastrulation and is enclosing.		enclosing embryo Ce	WBls:0000013
	The C. elegans life stage spanning 100-290min after first cleavage at 20 Centigrade. Proliferate from 28 cells to 421 cells. Referring to the whole period of gastrulation.		gastrulating embryo Ce	WBls:0000010