|
|
|
Expr12704
|
GFP::LEP-2 is expressed throughout development from embryo to adult. It is seen in many tissues, including seam-, tail tip- and other hypodermal cells, head and tail neurons, pharynx, intestine, and the developing hermaphroditic somatic gonad. Expression seems to be excluded from body muscle cells. |
The reporter is diffusely expressed in the cytoplasm but absent from nuclei. |
|
|
|
Expr14443
|
A Plep-5::GFP reporter was expressed in larvae of both sexes in several cell types including the tail tip, pharynx, nervous system, vulva, and seam cells. Notably, expression of the reporter was temporally controlled: GFP was first weakly detectable in early L2, became stronger in late L2, and persisted until late L3. Expression was weak in L4 animals and nearly undetectable in adults. |
|
|
|
|
Expr14445
|
lep-5 RNA was readily detectable in the tail tip of an L2 male. Co-staining with DAPI indicated that lep-5 RNA is predominantly, if not exclusively, cytoplasmic. Consistent with the broad expression of the transcriptional reporter, lep-5 RNA was also detectable throughout the body, including neuronal ganglia of the head and tail and in the developing male somatic gonad. |
|
|
|
|
Expr11057
|
During tail tip morphogenesis (mL4 = mid-L4), foci of NMY-2::GFP are observed at the apical surfaces of the migrating tail tip cells. An NMY- 2::GFP cap forms at the posterior surface of the migrating tail tip cells, and later (lL4 = late L4) along the ventral surface of the entire male tail. |
|
|
|
|
Expr14446
|
dmd-3 expression follows a characteristic temporal pattern, with expression first detectable in the tail tip cells in early L4. By late L4, TTM is complete and tail tip expression of dmd-3 becomes undetectable. |
|
|
|
|
Expr11059
|
The inx-12 reporter is expressed in tail tip cells prior to and during tail tip morphogenesis. inx-12 (YFP) is expressed at a low level in hyp8-11 prior to tail tip morphogenesis but more highly during retraction. |
|
|
|
|
Expr11060
|
The inx-13 reporter is expressed in tail tip cells prior to and during tail tip morphogenesis. inx-13 (YFP) is expressed at a low level in hyp8-11 prior to tail tip morphogenesis but more highly during retraction. |
|
|
|
|
Expr16422
|
An mtre-1::GFP transcriptional reporter is expressed downstream of MAB-3/DMD-3 activity in the male tail tip hypodermal cells for a brief period of time at the very end of retraction.Interestingly, transcriptional GFP reporter transgenes for mtre-1 are preferentially expressed in L4 male tail tips at the end of tail tip retraction. Specifically, 75% (27/36) of wild-type L4 males displayed high levels of mtre-1::GFP expression at the end of tail tip retraction, 19% (7/36) displayed low levels of expression and 6% (2/36) failed to show expression. In contrast, 81% (30/37) of wild-type L4 hermaphrodites failed to show any mtre-1::GFP expression in the tail tip, with the remaining 19% (7/37) only showing faint expression. To more closely examine the dynamics of mtre-1::GFP expression, we followed single wild-type L4 males throughout the process of retraction. We observe that mtre1::GFP expression in the male tail tip is restricted to a short 3- to 4-hour window that initiates after tail tip retraction is almost completed and terminates at the onset of anterior tail retraction. In contrast, we did not observe high levels of expression in wild-type L4 hermaphrodites at any time point. In addition, we did not observe any other major regions of expression of mtre1::GFP in either L4 males or hermaphrodites. |
|
|
Temporal description |
|
Expr11493
|
A CDC-42::GFP fusion protein is diffusely distributed in the cytoplasm of the tail tip cells at the early L4 stage and then localizes to foci at the apical surfaces of the tail tip cells just before retraction begins at mid-L4. During retraction, CDC-42::GFP again becomes diffuse in the cytoplasm. |
CDC-42::GFP is cytoplasmic prior to tail tip retraction, localizes to the membranes and discrete puncta during retraction (arrow), and becomes cytoplasmic again in late L4 and adults |
|
|
|
Expr11494
|
|
RME-8::GFP is localized in small round structures (which we interpret as vesicles) that form at the apical surfaces of the detaching tail tip cells and are concentrated at the cell surfaces during retraction. |
