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Expr12603
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sem-2 expression was identified in several early blastomeres and in some neuronal and non-neuronal progenitors. Its expression colocalized with that of sox-2 in a few progenitors. As in vertebrates, sem-2 expression was observed in postmitotic neurons, but in contrast to the broad expression of SoxC genes in vertebrate postmitotic neurons, only a single class of postembryonic neurons expresses sem-2, the RMH class. sem-2 expression in these neurons is observed throughout larval and adult stages. We did not detect any sem-2 expression in the RME neurons themselves, but sem-2 is instead expressed in the progenitor of RMEL/R. |
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Expr11853
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VBH-1 antibody staining showed the same localization in oocytes and embryos as observed with the VBH-1:GFP fusion (Expr11852). VBH-1 was observed throughout both the hermaphrodite and male gonad. In the male gonad, VBH-1 was observed in primary and secondary spermatocytes but not detected in mature sperm. |
VBH-1 was expressed in P granules throughout the gonad, and also diffusely in the cytoplasm. VBH-1, however, was not detected in the P-body-like particles. |
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Expr11852
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Transgenic animals showed VBH-1:GFP localization in the cytoplasm of both somatic and germline blastomeres during embryogenesis and in oocytes. |
VBH-1:GFP was observed in foci within the germline and co-localized with the P granule component PGL-1. VBH-1 localization was not consistently observed in every P granule, particularly during oogenesis, and on occasion we observed that PGL-1 and VBH-1 overlap only partially. |
