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Expr12480
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GFP fluorescence in animals carrying the unc-23::gfp construct is found throughout development appearing first at the 1.5-fold stage of embryogenesis in a few unidentified cells. Expression of the GFP-tagged UNC-23 protein continues during embryogenesis and by the 3-fold stage, it has expanded to include muscle, hypodermal and pharyngeal cells. In adult hermaphrodites GFP fluorescence is expressed in the pharynx, body wall muscle cells, hypodermis, vulva, and H cells, as well as some unidentified neurons and touch cells. In the hypodermis, UNC-23::GFP is distributed throughout the entire tissue including some nuclei and nucleoli, and in addition, is localized in a pattern reminiscent of the intermediate filaments. In body wall muscle cells, UNC-23::GFP is localized to the dense bodies and M lines in a pattern similar to that observed for several proteins including PAT-3/b-integrin, UNC-112, UNC-97/ PINCH, and PAT-4/ILK. |
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