1 Genes
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00000845 | cup-4 | C02C2.3 | Caenorhabditis elegans |
| Primary Identifier | Expr3280 | Subcellular Localization | In living worms, CUP-4::GFP localizes primarily to vesicles scattered around the cytoplasm. While some of the CUP-4::GFP localized around the nucleus, presumably in the endoplasmic reticulum (ER) membrane, the general pattern of localization of CUP-4::GFP was quite distinct from that of cytochrome b5, a marker for smooth ER, and from TRAM, a marker for the rough ER. To ascertain whether CUP-4 exits the ER, the transgene expressing CUP-4::GFP was crossed into a cup-5(ar465) background in which endocytosed material is not degraded and accumulates in large vacuoles. CUP-4::GFP, but neither of the ER markers, localized to the membrane of the large vacuoles. These results are consistent with CUP-4::GFP localizing at steady state to the Golgi apparatus or to the plasma membrane/endosomes. The residual ER accumulation of CUP-4::GFP in the cup-4(+) background is eliminated in a cup-4(ok837) background, suggesting that slight overexpression increases retention in the ER. |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00000845 | cup-4 | C02C2.3 | Caenorhabditis elegans |
