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Expr16149
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Focusing on larval development, we found that LIN-12::mNG::3xFlag was expressed in all postembryonic mesoderm progenitor cells at the 4M and 8M stages, but was only localized to the nucleus in ventral M-lineage cells, consistent with functions in dorsoventral patterning of the M lineage (Foehr & Liu, 2008). LIN-12::mNG::3xFlag was subsequently visible in somatic gonad cells and in several PN.p cells where lin-12 regulates cell fates (reviewed by Sternberg, 2005). LIN-12::mNG::3xFlag was visible at the cell membrane and in internalized punctae in the P3.p - P8.p cells but localized to the nucleus only in P5.p and P7.p. Following vulval precursor cell patterning, LIN-12::mNG::3xFlag was highly expressed in numerous somatic gonad cells, with nuclear localization in several spermatheca and uterine precursor cells. |
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Expr16540
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Animals carrying kpIs106 expressed GVD-1::GFP in various somatic tissues including the somatic gonad, vulva and seam cells. Subcellularly, GVD-1::GFP prominently localized to the nucleus but could be observed in the cytoplasm as well. GVD-1::GFP expression in the somatic gonad started at the L1 stage and persisted until the adult stage; its expression was clearly visible in Z1 and Z4, their progeny and the SGP at the L1, L2 and early L3 stages, respectively. In the vulval lineage, GVD-1::GFP was present in P6.p and its descendants. In late L3 and early L4 larvae, we noticed GVD-1::GFP in the spermathecal/uterine precursors. In adults, GVD-1::GFP could be observed in DTCs, Sh cells and spermatheca. GVD-1::GFP is seen in the developing uterus and vulva at the L3 stage, persisting in the uterus and vulval epithelium (VE) at the L4 stage. |
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Expr15692
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The transcriptional nlp-26 reporter was dynamically expressed in the VPCs and their sister Pn.a neurons in the VNC. In early to mid-L2 larvae, Pnlp-26-nls::lacZ::gfp was expressed in all VPCs and their sister Pn.a neurons. In addition, nlp-26 was strongly expressed in the hyp7 cell at all stages. By the late L2/early L3 stage, nlp-26 transcription was upregulated in P6.p and the P6.a neurons, while expression faded in the other VPCs. nlp-26 continued to be expressed in the P6.px daughter cells of mid-L3 larvae. Note that descendants of the 3° VPCs P3.p, P4.p and P8.p began to express nlp-26 after they had fused with hyp7. |
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Expr13669
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lin-39::lacZ was frequently expressed in P6.p to P8.p and at lower levels also in P3.p to P5.p of late L2 stage animals. |
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Expr15941
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LAG-1::mKate2, revealed a dynamic expression pattern in the VPCs during vulval induction. In order to achieve a more quantitative analysis, and potentially reveal more subtle dynamics, we quantitated LAG-1::GFP fluorescence, as GFP appeared brighter overall than mKate2. We observed that both LAG-1::GFP and LAG-1::mKate2 accumulated to a higher level in P5.p and P7.p compared with other VPCs (secondary fate pattern). This accumulation pattern is consistent with LIN-12 activation in the VPCs and suggests a positive feedback mechanism. |
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Expr15705
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In late L2/early L3 larvae before the VPCs have started dividing, a translational LET-23::GFP reporter was up-regulated in the 1 ̊ VPC P6.p, while expression faded in the other, more distal VPCs. Most of the LET-23:: GFP protein in P6.p was detected, approximately at equal levels, on the basolateral and apical plasma membranes. Only a faint and diffuse intracellular LET-23::GFP signal could be observed in the VPCs of wild-type animals. After the first round of VPC divisions, LET-23::GFP continued to be strongly expressed on the plasma membrane of the 1 ̊ P6.p descendants. |
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