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Expr16149
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Focusing on larval development, we found that LIN-12::mNG::3xFlag was expressed in all postembryonic mesoderm progenitor cells at the 4M and 8M stages, but was only localized to the nucleus in ventral M-lineage cells, consistent with functions in dorsoventral patterning of the M lineage (Foehr & Liu, 2008). LIN-12::mNG::3xFlag was subsequently visible in somatic gonad cells and in several PN.p cells where lin-12 regulates cell fates (reviewed by Sternberg, 2005). LIN-12::mNG::3xFlag was visible at the cell membrane and in internalized punctae in the P3.p - P8.p cells but localized to the nucleus only in P5.p and P7.p. Following vulval precursor cell patterning, LIN-12::mNG::3xFlag was highly expressed in numerous somatic gonad cells, with nuclear localization in several spermatheca and uterine precursor cells. |
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[egl-17::GFP] translational fusion. Contains 3.9 kb of 5'UTR, the full-length EGL-17 sequence and 3'UTR. |
Expr1434
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GFP expression in ayIs4[egl-17::GFP] hermaphrodites is first observed in two large unidentified cells in the head at the 3-fold stage of embryogenesis. After hatching, expression in these cells fades and is then observed in the M4 pharyngeal neuron where it remains throughout postembryonic development. Weak GFP expression can occasionally be observed in the ventral hypodermis in late first stage larvae (L1). This weak expression disappears by the L1 lethargus and was not characterized further. Strong GFP expression is observed in the VPCs and their descendants in two phases starting in late L2 and persisting through adulthood. During early vulval development (late L2-L3), expression is observed in P6.p and its descendants, fading in the early L4. In the mid L4, GFP expression is seen in the N and T descendants of P5.p and P7.p where it remains throughout adulthood. |
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Expr15692
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The transcriptional nlp-26 reporter was dynamically expressed in the VPCs and their sister Pn.a neurons in the VNC. In early to mid-L2 larvae, Pnlp-26-nls::lacZ::gfp was expressed in all VPCs and their sister Pn.a neurons. In addition, nlp-26 was strongly expressed in the hyp7 cell at all stages. By the late L2/early L3 stage, nlp-26 transcription was upregulated in P6.p and the P6.a neurons, while expression faded in the other VPCs. nlp-26 continued to be expressed in the P6.px daughter cells of mid-L3 larvae. Note that descendants of the 3° VPCs P3.p, P4.p and P8.p began to express nlp-26 after they had fused with hyp7. |
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Expr13304
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Expression of the lst-5pro::YFP Notch activity reporter is often surprisingly asymmetric, with one of the two 2 fate VPCs sometimes exhibiting 10-fold higher fluorescence intensities than the other. P5.p and P7.p were equally likely to show stronger reporter expression. Asymmetric reporter activity was also observed during the two-cell stage of vulval development. In 9 out of 27 animals, daughters of the less bright VPC at the one-cell stage became the brighter pair of cells. |
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Expr13669
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lin-39::lacZ was frequently expressed in P6.p to P8.p and at lower levels also in P3.p to P5.p of late L2 stage animals. |
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Expr15941
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LAG-1::mKate2, revealed a dynamic expression pattern in the VPCs during vulval induction. In order to achieve a more quantitative analysis, and potentially reveal more subtle dynamics, we quantitated LAG-1::GFP fluorescence, as GFP appeared brighter overall than mKate2. We observed that both LAG-1::GFP and LAG-1::mKate2 accumulated to a higher level in P5.p and P7.p compared with other VPCs (secondary fate pattern). This accumulation pattern is consistent with LIN-12 activation in the VPCs and suggests a positive feedback mechanism. |
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