WormMine: ExpressionPattern Expr13007

• Primary Identifier: Expr13007
• Remark: cp65, mrck-1(cp65[mrck-1::YPet + LoxP]) V
• Subcellular Localization: MRCK-1::YPet was enriched in the apical cortex specifically in the gastrulating endoderm precursor cells (EPCs). A GFP-tagged strain constructed similarly showed similar localization and allowed to co-visualize MRCK-1 and an mCherry-tagged PH domain to show membranes, revealing in ventral views that MRCK-1::GFP localized to puncta across the medio-apical surfaces of EPCs as well as to apical cell-cell borders. Fluorescently tagged MRCK-1 was not enriched in the apical domain of other early embryonic interphase cells, although some enrichment could be seen in the cortex of dividing cells. In the gastrulating EPCs, enrichment was detected starting from 5 min prior to MSx division (MSx indicating the two daughters of the MS cell), and within 15 min it had reached 2-fold enrichment over cytoplasmic levels. This was significantly different from non-dividing, non-internalizing AB cells, where little enrichment over cytoplasmic levels was detected in the same time frame. Comparison of the time course of apical constriction and MRCK-1 localization shows that apical enrichment of MRCK-1::YPet is concurrent with or even precedes apical constriction and continues throughout the gastrulation movements of the EPCs.

2 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
embryonic cell	Ep		WBbt:0006612
embryonic cell	Ea		WBbt:0006733

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00006437	mrck-1	K08B12.5	Caenorhabditis elegans

1 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The C. elegans life stage spanning 100-290min after first cleavage at 20 Centigrade. Proliferate from 28 cells to 421 cells. Referring to the whole period of gastrulation.		gastrulating embryo Ce	WBls:0000010