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No detailed description on cellular expression pattern at hermaphrodites. |
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Expr1300
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LIN-29 accumulates in B cell progeny. In contrast to the LIN-29 accumulation pattern observed in wild-type hermaphrodites, LIN-29 is not detectable in the F, U, Y, or M blast cell nuclei, or in their progeny, in males. LIN-29 is detected in the progeny of B. LIN-29 is first detectable in B cell progeny during the L3 stage. Although all B.a and B.p progeny accumulate LIN-29, the LIN-29 accumulation signals appear weaker and transient in B.p progeny. The identification of these LIN-29-accumulating cells as B progeny is based on their size, shape, and relative position within the male tail and is further supported by laser microsurgery experiments and the examination of LIN-29-accumulation patterns in a mutant with defects in B cell specification. Elimination of the B cell by laser micro-surgery during the L1 stage reduced the number of LIN-29-accumulating nuclei in the male tail by 1015. LIN-29 appears to persist in B.a progeny in the adult stage. LIN-29 accumulates in the linker cell. The first male-specific LIN-29 accumulation is detected in the nucleus of the linker cell (LC) positioned at the tip of the growing end of the gonad. LIN-29 accumulates in the LC during the L3 stage, after the gonad arm has completed the 180 turn. LIN-29 remains detectable until the late L4 stage when its disappearance is presumably due to LC destruction. LIN-29 accumulates in the male tail seam. LIN-29 accumulates in the lateral body seam cell nuclei and in the SET nuclei in L4 stage wild-type males. LIN-29 accumulates in ventral cord nuclei. During the late L3 stage, five to seven nuclei that belong to the preanal ganglion accumulate LIN-29. Four preanal ganglion nuclei accumulates LIN-29 and were identified by position as the CA9, CP9, AS11, and VA11 neurons which are descended from P11.a. LIN-29 accumulation in the preanal ganglion cluster persists through adulthood. In late L4 stage and adult males, additional ventral cord nuclei anterior to the preanal ganglion accumulate LIN-29. The accumulation of LIN-29 in ventral cord neurons is not observed in hermaphrodites. Also in contrast to hermaphrodites, the ventral hypodermal nuclei positioned within the ventral cord do not contain detectable levels of LIN-29 in adult males. |
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Expr13313
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evl-20 is widely expressed throughout the animal, including in P12.pa, B.alapaav and B.arapaav. |
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Expr13314
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The egl-1 reporter was expressed strongly in the B.al/rapaav cell fated to die and not at all or only weakly in the B.al/rapaav homolog, which is fated to live. All B.al/rapaav corpses in fourth larval stage wild-type animals were GFP- positive (n = 53), confirming that the GFP expression is associated with the secondary fate of cell death. |
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Expr13315
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We used single-molecule fluorescent in situ hybridization (smFISH) to determine whether, like the Pegl-1::4xNLS::gfp reporter (Expr13314), endogenous cell-death genes are transcribed in the dying B.al/rapaav. We usually detected egl-1 mRNA expression in B.al/rapaav and only rarely in the B.al/rapaav homolog, consistent with the expression pattern of Pegl-1::4xNLS::gfp. |
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Expr13316
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ced-3 transcripts were visible in only a subset of cells, usually including B.al/rapaav, and only rarely in the B.al/rapaav homolog. |
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