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Expr1853
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During larval development, tlp-1::gfp expressed in the posterior intestinal cells, several neuronal nuclei in the head, the tail tip cells in hermaphrodites and males and in descendants of the T cell lineage. Weak GFP expression observed in the T cell in 6% (n=124) of animals. After the division of the T cell, stronger GFP expression was observed in the posterior T cell daughter, T.p. but not in T.a. More specifically, of the animals that showed GFP expression in the T cell lineage (57/150), 88% of the animals examined showed GFP expression in T.p alone and 12% showed expression in T.a and T.p. GFP expression was also observed in the posterior T.ap daughter cell, T.app but not in T.ap or its anterior daughter cell T.apa. Specifically, all of the animals that showed expression in the T.ap lineage (11/56) showed GFP expression in T.app alone. No expression observed in T.apa in any animals. tlp-1::gfp expression begins to be barely detectable at the beginning of gastrulation at about 100 minutes of embryonic development. Shortly after the gastrulation begins, the level of expression increases and is detectable in the nuclei of most embryonic cells. This pattern appears to persist through gastrulation. However, at about 260 minutes, expression in the anterior of the embryo fades and expression in the posterior of the embryo persists and gets stronger. At the 1.5-fold stage, at about 400 minutes, expression was observed in posterior nuclei and this pattern continues throughout the rest of development. |
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