WormMine: ExpressionPattern Expr10542

• Primary Identifier: Expr10542
• Subcellular Localization: At the onset of cytokinesis, as the furrow has formed, cortical anterior PAR-6-mCherry fluorescence intensity decreases while it increases at the cleavage furrow. The anterior levels of PAR-6- mCherry then steadily decrease at beginning of furrow closure. In the posterior cortex of the single-cell embryo, PAR- 2-GFP fluorescence intensity decreases at furrow initiation to furrow closure, yet increases in intensity at the cleavage furrow. The posterior cortex fluorescence of PAR-2- GFP continues to decrease after furrow closure. After 4-min postclosure, posterior PAR-2-GFP fluorescence starts to increase until 16-min postclosure resulting in an 18% increase in cortical fluorescence intensity. The increase in the cortical posterior fluorescence correlates with the clearance of PAR-2-GFP at the cleavage furrow. In wild-type embryos, PAR-6-mCherry and PAR-2-GFP localize to the anterior and posterior poles prior to cleavage furrow initiation. As the furrow starts to ingress (2.7 min), both anterior and posterior PAR proteins appear along the furrow membrane, but only PAR-6-mCherry is found at the leading edge of the furrow membrane. As the furrow continues to ingress (4.7 min), this localization pattern is maintained. At 12.5 min after the initiation of furrow ingression, the flanking membrane along the furrow is predominately labeled with PAR-6- mCherry. The midbody region is the only place where PAR-2-GFP co-localizes with PAR-6-mCherry. As the embryo continues to divide to become a four-celled embryo, the localization of the anterior and posterior PAR proteins segregate to the membrane boundaries on opposite sides of the embryo. Here, PAR-6 is found predominantly along the membrane boundaries between the ABa, Abp, and EMS blastomeres. Anterior and posterior PAR protein localization at the furrow is observed until 8-min postcleavage furrow closure. Between 8- and 16-min postcleavage furrow closure, PAR-2-GFP localization becomes restricted to the membrane region in vicinity of the midbody (8-16-min postclosure). PAR-6-mCherry localizes along the entire furrow membrane during cytokinesis, whereas, PAR-2 is temporally and spatially restricted along the furrow.

0 Anatomy Terms

2 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00003917	par-2	F58B6.3	Caenorhabditis elegans
WBGene00003921	par-6	T26E3.3	Caenorhabditis elegans

0 Life Stages